L-Homopropargyl Glycine
(Synonyms: (S)-2-氨基-5-己炔酸,Click Tag? L-Homopropargyl Glycine,HPG) 目录号 : GC14450A methionine analog for click chemistry
Cas No.:98891-36-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L-homopropargylglycine (Hpg), an alkyne-tagged analog of methionine, has been used to selectively tag newly synthesized mammalian proteins in a method similar to conventional pulse-chase labeling, and labeled proteins were visualized via fluorescence. Protein translation has been implicated in different forms of synaptic plasticity, but direct in situ visualization of new proteins is limited to one or two proteins at a time. Homopropargylglycine is a noncanonical amino acid which can incorporate into proteins followed by chemoselective fluorescence tagging by means of 'click chemistry'. Pulse-chase application of homopropargylglycine allowed visualization of proteins synthesized in two sequential time periods. This technique can be used to detect changes in protein synthesis and to evaluate the fate of proteins synthesized in different cellular compartments [1].
Reference:
[1] Dieterich D C, Hodas J J L, Gouzer G, et al. In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons[J]. Nature neuroscience, 2010, 13(7): 897-905.
| Cas No. | 98891-36-2 | SDF | |
| 别名 | (S)-2-氨基-5-己炔酸,Click Tag? L-Homopropargyl Glycine,HPG | ||
| 化学名 | (2S)-2-amino-5-hexynoic acid | ||
| Canonical SMILES | C#CCC[C@H](N)C(O)=O | ||
| 分子式 | C6H9NO2 | 分子量 | 127.1 |
| 溶解度 | ≤10mg/ml in PBS(pH7.2) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 7.8678 mL | 39.3391 mL | 78.6782 mL |
| 5 mM | 1.5736 mL | 7.8678 mL | 15.7356 mL |
| 10 mM | 0.7868 mL | 3.9339 mL | 7.8678 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。