λ-Carrageenan
(Synonyms: 角叉胶,Gelatin) 目录号 : GC19401
λ-Carrageenan是一种存在于红藻细胞间质中的半乳聚糖多糖。λ-Carrageenan具有多样化的生物活性，例如抗凝血、抗病毒、抗血栓、抗菌、降胆固醇、抗肿瘤、免疫调节以及降高脂血症等功效。λ-Carrageenan还被用于治疗胃溃疡，并且具有抗氧化特性。
Cas No.:9064-57-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
λ-Carrageenan is a galactan polysaccharide that exists in the intercellular matrix of red algae. λ-Carrageenan has a variety of bioactivities, such as anticoagulant, antiviral, antithrombotic, antibacterial, cholesterol - lowering, antitumor, immunomodulatory, and antihyperlipidemic effects. λ-Carrageenan is also used for treating stomach ulcers and has antioxidant properties[1].
In vitro, λ-Carrageenan (0, 10, 50 and 100μg/ml; 12h or 1h) dose-dependently decreased the endocytosis of dendritic cells (DCs), promoted DC maturation and increased cytokine production through TLR4 mediated signaling pathway[2]. When the cultured human L - cells (NCI - H716) were deprived of glucose and serum overnight and then exposed to high glucose, 10% fetal bovine serum, and λ-Carrageenan (1µg/ml; 10min, 1h, and 24h), the mRNA expression of proglucagon and the secretion of glucagon-like peptide - 1 (GLP - 1) in these cells were significantly reduced compared with the control cells that were not exposed to λ-Carrageenan[3].
In vivo, Oral administration of λ-Carrageenan (0.001%; 16 days; p.o.) can alleviate ear swelling in hapten-induced atopic dermatitis model mice and significantly inhibit mast cell hyperplasia in the local skin[4]. λ-Carrageenan (50mg/kg; 20 days) inhibits tumor growth in B16-F10 and 4T1 bearing mice[5].
References:
[1] Ha HT, Cuong DX, Thuy LH, et al. Carrageenan of Red Algae Eucheuma gelatinae: Extraction, Antioxidant Activity, Rheology Characteristics, and Physicochemistry Characterization. Molecules. 2022 Feb 14;27(4):1268.
[2] Li J, Aipire A, Li J, et al. λ-Carrageenan improves the antitumor effect of dendritic cellbased vaccine. Oncotarget. 2017 May 2;8(18):29996-30007.
[3] Bhattacharyya S, Borthakur A, Tobacman JK. Common food additive carrageenan inhibits proglucagon expression and GLP-1 secretion by human enteroendocrine L-cells. Nutr Diabetes. 2024 May 16;14(1):28.
[4] Iwasaki T, Watarai S. Oral λ-carrageenan intake alleviates skin symptoms in a hapten induced atopic dermatitis-like model. J Vet Med Sci. 2020 Dec 5;82(11):1639-1642.
[5] Luo M, Shao B, Nie W, et al. Antitumor and Adjuvant Activity of λ-carrageenan by Stimulating Immune Response in Cancer Immunotherapy. Sci Rep. 2015 Jun 22;5:11062.
λ-Carrageenan是一种存在于红藻细胞间质中的半乳聚糖多糖。λ-Carrageenan具有多样化的生物活性，例如抗凝血、抗病毒、抗血栓、抗菌、降胆固醇、抗肿瘤、免疫调节以及降高脂血症等功效。λ-Carrageenan还被用于治疗胃溃疡，并且具有抗氧化特性[1]。
在体外实验中，λ-Carrageenan（0, 10, 50和100μg/ml; 12小时或1小时）剂量依赖性地降低了树突状细胞（DCs）的吞噬作用，通过TLR4介导的信号通路促进DC成熟并增加细胞因子的产生[2]。当培养的人L细胞（NCI-H716）在过夜剥夺葡萄糖和血清后暴露于高葡萄糖、10%胎牛血清以及λ-Carrageenan（1µg/ml; 10分钟, 1小时和24小时），与未暴露于λ-Carrageenan的对照细胞相比，这些细胞中胰高血糖素原的mRNA表达以及胰高血糖素样肽-1（GLP-1）的分泌显著降低[3]。
在体内实验中，口服λ-Carrageenan（0.001%; 16天; 口服）可以减轻致敏物诱导的特应性皮炎模型小鼠的耳部肿胀，并显著抑制局部皮肤中肥大细胞的增殖[4]。λ-Carrageenan（50mg/kg; 20天）可抑制B16-F10和4T1荷瘤小鼠的肿瘤生长[5]。
| Cell experiment [1]: | |
Cell lines | HCC1937 and BT-549 cells |
Preparation Method | Dendritic cells (DCs) were derived from murine bone marrow cells. On day 7, immature DCs were collected and treated with different concentrations (10, 50 and 100μg/ml) of λ-Carrageenan. Lipopolysaccharide (LPS) at 20ng/ml was used as a positive control. For Toll-like receptor 4 (TLR4) inhibitor experiments, immature DCs were pre-treated with 1μM TAK-242 (TLR4 inhibitor) for 1h, and then treated with 0, 10, 50 and 100μg/ml of λ-Carrageenan or 20ng/ml of LPS for 12h. After treatment, supernatants and cells were collected to analyze cytokine secretion by enzyme-linked immunosorbent assay (ELISA) and DC maturation by flow cytometry, respectively. For endocytosis analysis, DCs treated with λ-Carrageenan were incubated with 50μg/ml FITC-Dextran at 37°C for 1h. After washing with cold PBS, the cells were stained with APC-CD11c and analyzed by flow cytometry. |
Reaction Conditions | 0, 10, 50 and 100μg/ml; 12h or 1h |
Applications | λ-Carrageenan dose-dependently decreased the endocytosis of dendritic cells (DCs), promoted DC maturation and increased cytokine production through TLR4 mediated signaling pathway. |
| Animal experiment [2, 3]: | |
Animal models | Male adult C57BL/6J mice |
Preparation Method | A total volume of 25-30μL of 0.1% λ-Carrageenan solution dissolved in saline (at 60℃) and cooled further was subcutaneously injected into the mice through the plantar surface of the left hind paw. The paw size was measured at 1, 2, 3 and 4h after the injection of λ-Carrageenan. Edema was expressed as the percentage of the difference between the paw size after (at respective time points) and before (basal values) the injection of λ-Carrageenan. |
Dosage form | 25-30μL of 0.1%; 1, 2, 3 and 4h; s.c. |
Applications | λ-Carrageenan-induced inflammatory mouse model |
References: | |
| Cas No. | 9064-57-7 | SDF | |
| 别名 | 角叉胶,Gelatin | ||
| 化学名 | [5-[4,5-dihydroxy-6-(hydroxymethyl)-3-sulfonatooxyoxan-2-yl]oxy-2,4-dihydroxy-6-(sulfonatooxymethyl)oxan-3-yl] sulfate | ||
| Canonical SMILES | C(C1C(C(C(C(O1)OC2C(OC(C(C2O)OS(=O)(=O)[O-])O)COS(=O)(=O)[O-])OS(=O)(=O)[O-])O)O)O | ||
| 分子式 | 分子量 | ||
| 溶解度 | 25 mg/mL in water (ultrasound assisted dissolution) | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch: