Sample solution is provided at 25 µL, 10mM.
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The rats are fasted over night with free access to water. For stereotaxic injection, rats are anaesthetized (sodium pentobarbital, 50 mg/kg, i.p.) and immobilized in a Kopf stereotaxic frame. The rats are microinjected with 1 μg Kainic acid in 2 μL of sterilized physiological saline into the right lateral ventricle, i.e., 0.2 mm caudal from the bregma, 1.2 mm right lateral and 3.8 mm ventral according to the rat brain atlas. For control, rats are injected with same amount (2 μL) of vehicle, sterilized physiological saline. Five rats in each group are used in this study.
. Matsuoka Y, et al. Kainic acid induction of heme oxygenase in vivo and in vitro. Neuroscience. 1998 Aug;85(4):1223-33.
Kainic acid is a selective agonist of kainate receptor .
Kainate receptor is an ionotropic receptor that responds to glutamate. Presynaptic kainate receptor modulates GABA release and is involved in inhibitory neurotransmission. Postsynaptic kainate receptor is involved in excitatory neurotransmission.
In aged rats, kainic acid significantly reduced the latency to full clonic-tonic seizures and increased the amount of seizures rats. Also, kainic acid significantly increased the release of norepinephrine (NE), ASP and GLU in aged rats with clonic-tonic seizures . In neonatal rats, intrahippocampal injection of kainic acid (1 μg) significantly induced pyramidal cell death . In adult rats, kainic acid significantly increased the mRNA levels of neurotrophin-4/5 (NT-4/5) in the dorsal horn and in the spinal cord white matter, and increased the mRNA level of brain-derived neurotrophic factor (BDNF) in the ventral horn. While kainic acid didn’t affect neurotrophin-3 (NT-3). These results suggested that NT-4/5 and BDNF participated in the response of the spinal cord to excitotoxic stimuli induced by kainic acid .
. Dawson R Jr, Wallace DR. Kainic acid-induced seizures in aged rats: neurochemical correlates. Brain Res Bull, 1992, 29(3-4): 459-468.
. Cook TM, Crutcher KA. Intrahippocampal injection of kainic acid produces significant pyramidal cell loss in neonatal rats. Neuroscience, 1986, 18(1): 79-92.
. Scarisbrick IA, Isackson PJ, Windebank AJ. Differential expression of brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4/5 in the adult rat spinal cord: regulation by the glutamate receptor agonist kainic acid. J Neurosci, 1999, 19(18): 7757-7769.
|溶解度||≥ 11.05mg/mL in H2O with gentle warming||储存条件||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
|% DMSO % % Tween 80 % ddH2O|
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH2O，混匀澄清。
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