Inosinic acid (IMP)
(Synonyms: 5'-肌苷酸,5'-IMP; IMP; Inosine 5'-dihydrogen phosphate) 目录号 : GC19718
Inosinic acid (IMP) 是一种在嘌呤代谢中起核心作用的核苷酸，也是重要的食品风味增强剂。
Cas No.:131-99-7
Sample solution is provided at 25 µL, 10mM.
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Inosinic acid (IMP) is a nucleotide that plays a central role in purine metabolism and is also an important flavor enhancer in food[1]. Inosinic acid can be further converted into adenosine monophosphate (AMP) and guanosine monophosphate (GMP), and it participates in the energy metabolism process within cells[2]. Inosinic acid is commonly used as a food additive (E630), and when used in combination with monosodium glutamate (MSG), Inosinic acid significantly enhances the umami flavor of food and is widely used in feed additives[3]. Inosinic acid also shows potential research value in immune regulation and neuroprotection[4].
In vitro, treatment of lineage⁻/low bone marrow cells with Inosinic acid (15mM) for 15–30 minutes activates the pAkt and pS6 signaling pathways, stimulating the upregulation of cKit membrane expression and promoting the proliferation of granulocyte-macrophage progenitors (GMP) and the generation of Gr-1⁺ granulocytes[5].
In vivo, Inosinic acid (45mg/kg) was intraperitoneally injected 15 minutes after X-ray irradiation to treat male Kv:SHK strain mice that had undergone 7Gy total body irradiation. Inosinic acid significantly increased the survival rate after a lethal dose of irradiation, alleviated radiation-induced leukopenia and thrombocytopenia, and reduced the level of DNA damage in bone marrow and thymus cells[6]. Inosinic acid (1000mg/kg) was intraperitoneally injected into 10- to 12-week-old C57BL/6J mice with sepsis. Inosinic acid significantly inhibited TNF-α production in sepsis and enhanced IL-10 levels[7].
References:
[1] Dewulf JP, Marie S, Nassogne MC. Disorders of purine biosynthesis metabolism. Mol Genet Metab. 2022 Jul;136(3):190-198.
[2] Takahashi H, Tokura M, Kawarasaki S, et al. Metabolomics reveals inosine 5'-monophosphate is increased during mice adipocyte browning. J Biol Chem. 2022 Oct;298(10):102456.
[3] Zhang GQ, Ma QG, Ji C. Effects of dietary inosinic acid on carcass characteristics, meat quality, and deposition of inosinic acid in broilers. Poult Sci. 2008 Jul;87(7):1364-9.
[4] Duong-Ly KC, Kuo YM, Johnson MC, et al. T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly. J Cell Sci. 2018 Sep 5;131(17):jcs223289.
[5] Luo XM, Lam SM, Dong Y, et al. The purine metabolite inosine monophosphate accelerates myelopoiesis and acute pancreatitis progression. Commun Biol. 2022 Oct 12;5(1):1088.
[6] Asadullina NR, Usacheva AM, Gudkov SV. Protection of mice against X-ray injuries by the post-irradiation administration of inosine-5'-monophosphate. J Radiat Res. 2012;53(2):211-6.
[7] Lovászi M, Németh ZH, Gause WC, et al. Inosine monophosphate and inosine differentially regulate endotoxemia and bacterial sepsis. FASEB J. 2021 Nov;35(11):e21935.
Inosinic acid (IMP) 是一种在嘌呤代谢中起核心作用的核苷酸，也是重要的食品风味增强剂[1]。Inosinic acid可以进一步转化为腺苷酸（AMP）和鸟苷酸（GMP），并参与细胞内的能量代谢过程[2]。Inosinic acid常作为食品添加剂（E630）使用，与谷氨酸钠（MSG）协同作用可显著增强食物的鲜味（umami），广泛应用于饲料添加剂中[3]。Inosinic acid还在免疫调节和神经保护等方面显示出潜在的研究价值[4]。
在体外，Inosinic acid (15mM) 处理ineage⁻/low骨髓细胞15–30分钟，通过激活pAkt和pS6信号通路刺激cKit膜表达上调，并促进粒细胞-单核细胞祖细胞（GMP）增殖与Gr-1⁺粒细胞生成[5]。
在体内，Inosinic acid（45mg/kg）于X射线照射后15分钟腹腔注射，用于处理经7Gy全身照射的Kv:SHK品系雄性小鼠。Inosinic acid显著提高了致死剂量照射后的存活率，减轻了辐射引起的白细胞减少症和血小板减少症，并降低了骨髓和胸腺细胞中的DNA损伤水平[6]。Inosinic acid (1000mg/kg)腹腔注射于10-12周龄的内毒素血症C57BL/6J小鼠。Inosinic acid显著抑制了内毒素血症中的TNF-α产生，并增强了IL-10水平[7]。
| Cell experiment [1]: | |
Cell lines | Lineage-/low bone marrow cells (murine hematopoietic progenitor cells) |
Preparation Method | Lineage-/low cells were isolated from wild-type C57BL/6 mouse bone marrow using a lineage cell depletion kit. Cells were cultured in SFEM medium supplemented with 10ng/mL stem cell factor, 10ng/mL thrombopoietin, and 10ng/mL IL-3 at 37°C, 5% CO₂. Cells were treated with Inosinic acid at concentrations of 0-15mM for 24-72 hours. |
Reaction Conditions | 0-15mM; 24-72h. |
Applications | Inosinic acid treatment significantly stimulated granulocyte-monocyte progenitor (GMP) cell proliferation and Gr-1+ granulocyte production in a dose-dependent manner. Inosinic acid enhanced BrdU incorporation into GMP cells by 1.3-fold and increased the proportion of Gr-1+ cells by 1.4-fold compared to control cells. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | Mice were intraperitoneally administered Inosinic acid (1000mg/kg) 30 minutes prior to LPS (10mg/kg, i.p.) challenge. Plasma and organ samples (spleen, liver, lung) were collected 90 minutes post-LPS for cytokine analysis. For bacterial sepsis models, Inosinic acid (1000mg/kg, i.p.) was administered 1 hour before cecal ligation and puncture (CLP) or intranasal bacterial infection (Streptococcus pneumoniae and Escherichia coli). |
Dosage form | 1000mg/kg; i.p. |
Applications | Inosinic acid significantly suppressed TNF-α production and augmented IL-10 levels in endotoxemic mice. |
References: | |
| Cas No. | 131-99-7 | SDF | |
| 别名 | 5'-肌苷酸,5'-IMP; IMP; Inosine 5'-dihydrogen phosphate | ||
| Canonical SMILES | O[C@]([C@](O)([H])[C@]1([H])N2C=NC3=C2N=CN=C3O)([H])[C@@](O1)([H])COP(O)(O)=O | ||
| 分子式 | C10H13N4O8P | 分子量 | 348.21 |
| 溶解度 | insoluble in EtOH; >70 mg/mL in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8718 mL | 14.3592 mL | 28.7183 mL |
| 5 mM | 574.4 μL | 2.8718 mL | 5.7437 mL |
| 10 mM | 287.2 μL | 1.4359 mL | 2.8718 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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