IFN a 2b Human, Yeast
目录号 : GP20386Interferon-Alpha 2b Human Recombinant, Yeast
Sample solution is provided at 25 µL, 10mM.
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Interferon-alpha 2b Human Recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa.The Interferon-alpha 2b gene was obtained from human leukocytes.The IFN-a 2b is purified by proprietary chromatographic techniques.
| Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Saccharomyces cerevisiae. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Interferon alpha 2b; IFNA; INFA2; IFN-? 2b; MGC125764; MGC125765. | ||
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. | ||
| Solubility | It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. | ||
| Biological Activity | The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg. | ||
| Formulation | Lyophilized from a 0.2ж╠m filtered concentrated (1mg/ml) solution in PBS, pH-7.4. | ||
IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg.
Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.