HPSE Active

Heparanase is an endo β-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).

The specific activity of Heparanase Active Enzymein-house standard is about 0.7Units (1 unit = 1 μmole of reducing ends of heparan sulfatesubstrate formed per minute per mg Heparanase Active Enzymeat 37°C). Theenzymatic activity of each Heparanase Active Enzymebatch is comparable to thestandard as determined by activity assay in which immobilizedheparan, released due to heparanase activity, is quantifiedcolorimetrically. Recommended reaction buffer: 20 mM CitratePhosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2.