HBcAg [Hepatitis B virus] (18-27)
(Synonyms: H-Phe-Leu-Pro-Ser-Asp-Phe-Phe-Pro-Ser-Val-OH ) 目录号 : GP10047
Indicator of active viral replication
![HBcAg [Hepatitis B virus] (18-27) Chemical Structure](/media/struct/GP1/GP10047.png "HBcAg [Hepatitis B virus] (18-27) Chemical Structure")
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
HBcAg (core antigen) is a hepatitis B viral protein1,2. It is an indicator of active viral replication; this means the person infected with Hepatitis B can likely transmit the virus on to another person.HBeAg is the extracellular form of HBcAg, hence why the presence of both are markers of viral replication, and antibodies to these antigens are markers of a decline in replication.Multiple protein products can be produced from the same DNA sequence. When "ORF Core" and "Pre C" are translated together, the result is "HBeAg".Whereas HBcAg is considered "particulate", "HBeAg" is considered "nonparticulate" or "secretory".Both HBeAg and HBcAg are made from the same reading frame, but HBeAg is secreted from cells and accumulates in serum as an immunologically distinct soluble antigen. HBeAg is secreted and found in the serum of patients and serves as a marker of active replication in chronic hepatitis. Although the function of HBeAg is not clearly understood, one study demonstrated that it downregulated Toll-like receptor 2 expression on hepatocytes and monocytes leading to a decrease in cytokine expression. HBeAg is dispensable for replication, as mutant viruses with defects in the pre-C region are both infectious and pathogenic3.
References:
1. Kimura T, Rokuhara A, Matsumoto A, et al. (May 2003). "New enzyme immunoassay for detection of hepatitis B virus core antigen (HBcAg) and relation between levels of HBcAg and HBV DNA". J. Clin. Microbiol. 41 (5): 1901-6.
2. Cao T, Meuleman P, Desombere I, S?llberg M, Leroux-Roels G (December 2001). "In vivo inhibition of anti-hepatitis B virus core antigen (HBcAg) immunoglobulin G production by HBcAg-specific CD4(+) Th1-type T-cell clones in a hu-PBL-NOD/SCID mouse model". J. Virol. 75 (23): 11449-56.
3. Mandell,Douglas, and Bennett's Principles and Practice of Infectious Diseases. 7th ed. page 2062
| Cas No. | SDF | ||
| 别名 | H-Phe-Leu-Pro-Ser-Asp-Phe-Phe-Pro-Ser-Val-OH | ||
| Canonical SMILES | N[C@H](C(N[C@H](C(N1[C@@H](CCC1)C(N[C@@H](CO)C(N[C@H](C(N[C@@H](CC2=CC=CC=C2)C(N[C@@H](CC3=CC=CC=C3)C(N4[C@@H](CCC4)C(N[C@H](C(N[C@H](C(O)=O)C(C)C)=O)CO)=O)=O)=O)=O)CC(O)=O)=O)=O)=O)CC(C)C)=O)CC5=CC=CC=C5 | ||
| 分子式 | C58H78N10O15 | 分子量 | 1155.3 |
| 溶解度 | ≥ 115.5mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.8656 mL | 4.3279 mL | 8.6558 mL |
| 5 mM | 0.1731 mL | 0.8656 mL | 1.7312 mL |
| 10 mM | 0.0866 mL | 0.4328 mL | 0.8656 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。