HB-EGF Rat目录号 : GP20275
Proheparin-Binding EGF-like Growth Factor Rat Recombinant
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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|Purity||Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.||Source||Escherichia Coli.|
|Phycical Appearance||Sterile Filtered White lyophilized (freeze-dried) powder.||Shipping Condition||Shipped at Room temp.|
|Synonyms||Proheparin-binding EGF-like growth factor; Heparin-binding EGF-like growth factor; HB-EGF; HBEGF; Dtr; Hegfl; GFHB.|
|Amino Acid Sequence||DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL.|
|Solubility||It is recommended to reconstitute the lyophilizedRat HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.|
|Formulation||The protein was filtered (0.2µm) and lyophilized from a concentrated solution containing PBS, 300mM NaCl, pH 7.4 and 5% trehalose.|
HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGFбпs ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.
The ED50 as determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 units/mg.
LyophilizedRat HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.