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H-Ala-OH Sale

(Synonyms: L-丙氨酸; L-2-Aminopropionic acid) 目录号 : GA11068

H-Ala-OH 是一种非必需氨基酸,参与糖和酸代谢,提高免疫力,为肌肉组织、大脑和中枢神经系统提供能量。

H-Ala-OH Chemical Structure

Cas No.:56-41-7

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Sample solution is provided at 25 µL, 10mM.

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实验参考方法

Cell experiment:

To investigate the selective removal of hiPSCs from differentiated cells by the high L-Alanine medium, two types of hiPSCs, 201B7 hiPSCs (201B7 cells) and an hiPSC line derived by episomal system (ehiPSCs) are used, along with normal human dermal fibroblasts (hFBs), human skeletal muscle cells (hSkMCs) and hiPSC-derived cardiomyocytes (iCMs) as differentiated cells. The cells are incubated in a medium supplemented with L-Alanine at various concentrations (0-1.2 M) or treatment times (1-24 h). The medium is replaced with a normal medium and the relative cell viability is measured after 24 h[1].

References:

[1]. Nagashima T, et al. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine. Sci Rep. 2018 Aug 20;8(1):12427.

产品描述

L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.

The viability of both hiPSCs, 201B7 cells and ehiPSCs decrease with an increase in L-Alanine concentration, and reach 7.5±1.3% and 3.7±0.7% respectively at 1.2 M of L-Alanine. On the other hand, no decrease in the viability of hFBs and hSkMCs are observed. Although the viability of iCMs slightly decreases along with the increase of the L-Alanine concentration, viability of iCMs at 1.2 M concentration of L-Alanine, 49.4±6.9%, is significantly higher than that of undifferentiated iPSCs, 201B7 cells and ehiPSCs (p< 0.01). The viability of hiPSCs, 201B7 cells and ehiPSCs, drastically decrease even after 2 or 4 h treatment. In contrast, the viability of hFBs fails to decrease at 1, 2, and 4 h and shows a small decrease at 24 h treatment. The viability of 201B7 cells in suspension culture decreases to 11.8±6.0% following treatment with 1.2 M L-Alanine for 2 h, whereas that of hFBs is 72.9±14.2%[1].

References:
[1]. Nagashima T, et al. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine. Sci Rep. 2018 Aug 20;8(1):12427.

Chemical Properties

Cas No. 56-41-7 SDF
别名 L-丙氨酸; L-2-Aminopropionic acid
化学名 (2S)-2-aminopropanoic acid
Canonical SMILES CC(C(=O)O)N
分子式 C3H7NO2 分子量 89.1
溶解度 PBS (pH 7.2): 10mg/mL 储存条件 Desiccate at -20°C
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 11.2233 mL 56.1167 mL 112.2334 mL
5 mM 2.2447 mL 11.2233 mL 22.4467 mL
10 mM 1.1223 mL 5.6117 mL 11.2233 mL
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