Geninthiocin A
目录号 : GC43744
A cyclic thiopeptide bacterial metabolite
Cas No.:158792-27-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Geninthiocin A is a cyclic thiopeptide bacterial metabolite originally isolated from Streptomyces sp. DD84. It is active against a variety of Gram-positive bacteria (MICs = 0.2-4 µg/ml), the Gram-negative bacterium C. violaceum (MIC = 19 µg/ml), and the fungus M. hiemalis (MIC = 38 µg/ml). Geninthiocin A also induces tipA promoter transcription with a minimum induction concentration of 1.2 ng/ml.
| Cas No. | 158792-27-9 | SDF | |
| Canonical SMILES | O=C(N[C@]([C@@H](C)O)([H])C(N/C(C1=NC(C(NC2=C)=O)=CO1)=C\C)=O)C3=CSC(C4=C(C5=COC(C(NC(C(NC(C6=C(C)OC(C(NC([C@H](C(O)(C)C)NC2=O)=O)=C)=N6)=O)=C)=O)=C)=N5)N=C(C(NC(C(NC(C(N)=O)=C)=O)=C)=O)C=C4)=N3 | ||
| 分子式 | C50H49N15O15S | 分子量 | 1132.1 |
| 溶解度 | DMF: soluble,DMSO: soluble,Ethanol: slightly soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.8833 mL | 4.4166 mL | 8.8331 mL |
| 5 mM | 0.1767 mL | 0.8833 mL | 1.7666 mL |
| 10 mM | 0.0883 mL | 0.4417 mL | 0.8833 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Geninthiocins C and D from Streptomyces as 35-membered macrocyclic thiopeptides with modified tail moiety
J Antibiot (Tokyo) 2019 Feb;72(2):106-110.PMID:30479394DOI:10.1038/s41429-018-0127-y.
Geninthiocin is a thiopeptide with 35-membered macrocyclic core moiety. It has potent anti-Gram-positive (G+) bacteria activity. Herein, we reported two new congeners (2-3) of geninthiocin (Geninthiocin A, 1) from Streptomyces sp. CPCC 200267, and designated them as geninthiocins C and D, whose structures were determined by NMR. Geninthiocins A, C and D had the same 35-membered macrocyclic core moiety, but possessed a -Dha-Dha-NH2, -Dha-Ala-NH2, and -NH2 tail, respectively. Besides, the Ala residue in geninthiocin C was determined as L- configuration by C3 Marfey's method. In vitro assays indicated that geninthiocins C-D showed no antibacterial activity, in contrast to the potent anti-G+ bacteria activity displayed by Geninthiocin A. Therefore, the -Dha-Dha-NH2 tail of Geninthiocin A played an important role in its potent activity against G+ bacteria.
Geninthiocins E and F, two new cyclic thiopeptides with antiviral activities from soil-derived Streptomyces sp. CPCC 200267 using OSMAC strategy
J Antibiot (Tokyo) 2023 Feb;76(2):101-104.PMID:36434277DOI:10.1038/s41429-022-00580-0.
On the basis of the one strain-many compounds (OSMAC) strategy, two new cyclic thiopeptides, geninthiocins E and F, together with four known geninthiocin derivatives, geninthiocins A, B, C, and val-geninthiocin were isolated from Streptomyces sp. CPCC 200267. Their structures and absolute configurations were elucidated by extensive spectroscopic analyses and Marfey's method. Geninthiocin E (1), val-geninthiocin (3), Geninthiocin A (4), and geninthiocin B (5) exhibited significant anti-influenza A virus activities with the IC50 values of 28.7, 15.3, 7.3, and 18.3 μM, respectively. Compounds 3 and 4 showed moderate antibacterial activities against Staphylococcus aureus.