Ilexsaponin A
(Synonyms: 毛冬青皂苷A) 目录号 : GC38566Ilexsaponin A 是从冬凌草的根中分离出的,通过抗凋亡途径减轻缺血再灌注引起的心肌损伤。Ilexsaponin A 可以减少心肌梗塞的大小,降低 LDH,AST 和 CK-MB 的血清水平,增加细胞活力并抑制缺氧/复氧心肌细胞的凋亡。
Cas No.:108524-93-2
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
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Ilexsaponin A, isolated from the root of Ilex pubescens, attenuates ischemia-reperfusion-induced myocardial injury through anti-apoptotic pathway. Ilexsaponin A can reduce myocardial infarct size, lower the serum levels of LDH, AST and CK-MB, increase cellular viability and inhibit apoptosis in hypoxia/reoxygenation cardiomyocytes[1].
Ilexsaponin A significantly reduces proapoptotic proteins including caspase-3, cleaved caspase-3 and bax and increases anti-apoptotic protein bcl-2 in hypoxia/reoxygenation cardiomyocytes. Ilexsaponin A treatment increases the expression levels of p-Akt in hypoxia/reoxygenation cellular model and myocardial ischemia/reperfusion animal model[1].
Ilexsaponin A (10 or 40 mg/kg) reduce the myocardial infarct size in a dose dependent manner in male Sprague-Dawley rats weighing 280-320 g[1].
[1]. Zhang SW, et al. Ilexsaponin A attenuates ischemia-reperfusion-induced myocardial injury through anti-apoptotic pathway. PLoS One. 2017 Feb 9;12(2):e0170984.
Cas No. | 108524-93-2 | SDF | |
别名 | 毛冬青皂苷A | ||
Canonical SMILES | OC[C@H]([C@@H](O)[C@H](O)[C@H]1O)O[C@H]1OC([C@]23[C@]([C@](O)([C@H](C)CC3)C)([H])C4=CC[C@@]([C@@]5([C@@]([C@](C)([C@@H](O)CC5)C(O)=O)([H])CC6)C)([H])[C@]6(C)[C@]4(C)CC2)=O | ||
分子式 | C36H56O11 | 分子量 | 664.82 |
溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 1.5042 mL | 7.5208 mL | 15.0417 mL |
5 mM | 0.3008 mL | 1.5042 mL | 3.0083 mL |
10 mM | 0.1504 mL | 0.7521 mL | 1.5042 mL |
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Ilexsaponin A attenuates ischemia-reperfusion-induced myocardial injury through anti-apoptotic pathway
PLoS One 2017 Feb 9;12(2):e0170984.PMID:28182689DOI:10.1371/journal.pone.0170984.
The protective effects of Ilexsaponin A on ischemia-reperfusion-induced myocardial injury were investigated. Myocardial ischemia/reperfusion model was established in male Sprague-Dawley rats. Myocardial injury was evaluated by TTC staining and myocardial marker enzyme leakage. The in vitro protective potential of Ilexsaponin A was assessed on hypoxia/reoxygenation cellular model in neonatal rat cardiomyocytes. Cellular viability and apoptosis were evaluated by MTT and TUNEL assay. Caspase-3, cleaved caspase-3, bax, bcl-2, p-Akt and Akt protein expression levels were detected by western-blot. Ilexsaponin A treatment was able to attenuate the myocardial injury in ischemia/reperfusion model by reducing myocardial infarct size and lower the serum levels of LDH, AST and CK-MB. The in vitro study also showed that Ilexsaponin A treatment could increase cellular viability and inhibit apoptosis in hypoxia/reoxygenation cardiomyocytes. Proapoptotic proteins including caspase-3, cleaved caspase-3 and bax were significantly reduced and anti-apoptotic protein bcl-2 was significantly increased by Ilexsaponin A treatment in hypoxia/reoxygenation cardiomyocytes. Moreover, Ilexsaponin A treatment was able to increase the expression levels of p-Akt in hypoxia/reoxygenation cellular model and myocardial ischemia/reperfusion animal model. Coupled results from both in vivo and in vitro experiments indicate that Ilexsaponin A attenuates ischemia-reperfusion-induced myocardial injury through anti-apoptotic pathway.
[Determination of three pair of triterpenoid isomers in leaf of Ilex hainanensis by HPLC-ELSD]
Zhongguo Zhong Yao Za Zhi 2018 Apr;43(8):1662-1666.PMID:29751714DOI:10.19540/j.cnki.cjcmm.20180115.015.
The present study is to develop an HPLC-ELSD method for simultaneous determination of three pairs of triterpenoid isomers, Ilexsaponin A₁, Ilexhainanoside D, Ilexgenin A, 3β, 19α-dihydroxyolean-12-ene-24, 28-dioic acid (ilexhainanin E) ursolic acid and oleanic acid in the leaf of Ilex hainanensis, which could provide evidence to the quality control of this herb. The six constituents were measured on a Waters XBridge C₁₈ column (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of methanol (A)- 0.5% formic acid in water (B) at a flow rate of 1.0 mL·min⁻¹ (0-18 min,70%-85% A,18-20 min,85%-95% A;20-35 min,95% A). The carrier gas was N₂, and the pressure was 2.8 L·min⁻¹. The drift tube in this experiment were set at 70 °C. The injection volume was 10 μL. The contents of the six triterpenoids in 6 samples were 3.7-8.5, 10.3 -22.1, 2.8-5.9, 7.8-14.1, 2.6-3.8 and 8.8-11.9 mg·g⁻¹, respectively. The established method is proved to be accurate and sensitive for the determination of triterpenoids in Ilicis Hainanensis Folium, and may be used for the quality improvement of this herb.