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(Arg)9 Sale

(Synonyms: 九聚精氨酸,Nona-L-arginine; Peptide R9) 目录号 : GC34976

A cationic cell-penetrating peptide

(Arg)9 Chemical Structure

Cas No.:143413-47-2

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1mg
¥1,170.00
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5mg
¥4,410.00
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Sample solution is provided at 25 µL, 10mM.

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实验参考方法

Cell experiment:

(Arg)9 is prepared as 100× stocks (500 μM) in normal saline and assessed in a concentration range from 0.1 to 15 μM. (Arg)9 is added to culture 96-well plate 15 min prior to glutamic acid or kainic acid exposure. Neuronal viability is quantitatively using the MTS assay[1].

Animal experiment:

Rats: (Arg)9 (D-isoform) is prepared in 100× stocks (500 μM in water and assessed in a concentration range from 0.1 to 20 μM. Rats are fasted overnight and subjected to filament permanent middle cerebral artery occlusion (MCAO). Thirty minutes post-MCAO rats are intravenously treated with (Arg)9 (1 μmol/kg in 600 μL over 5 minutes) or vehicle (normal saline for injection; 600 μL over 5 minutes). Treatments are randomized and all procedures are performed masked to treatment. Twenty-four hours post-MCAO infarct area assessment is performed[2].

References:

[1]. Meloni BP, et al. The neuroprotective efficacy of cell-penetrating peptides TAT, penetratin, Arg-9, and Pep-1 in glutamic acid, kainic acid, and in vitro ischemia injury models using primary cortical neuronal cultures. Cell Mol Neurobiol. 2014 Mar;34(2):173-81.
[2]. Meloni BP, et al. Poly-arginine and arginine-rich peptides are neuroprotective in stroke models. J Cereb Blood Flow Metab. 2015 Jun;35(6):993-1004.
[3]. Milani D, et al. Poly-arginine peptides reduce infarct volume in a permanent middle cerebral artery rat strokemodel. BMC Neurosci. 2016 May 3;17(1):19.

产品描述

(Arg)9 is a cationic cell-penetrating peptide.1,2 It has been used to deliver various cargo to cells, including fluorescent proteins and plasmids. (Arg)9 (5 and 10 ?M) also protects primary rat cortical neurons from glutamate-induced excitotoxicity.3

1.Chang, M., Chou, J.-C., and Lee, H.-J.Cellular internalization of fluorescent proteins via arginine-rich intracellular delivery peptide in plant cellsPlant Cell Physiol.46(3)482-488(2005) 2.Lee, C.-Y., Li, J.-F., Liou, J.-S., et al.A gene delivery system for human cells mediated by both a cell-penetrating peptide and a piggyBac transposaseBiomaterials32(26)6264-6276(2011) 3.Meloni, B.P., Brookes, L.M., Clark, V.W., et al.Poly-arginine and arginine-rich peptides are neuroprotective in stroke modelsJ. Cereb. Blood Flow Metab.35(6)993-1004(2015)

Chemical Properties

Cas No. 143413-47-2 SDF
别名 九聚精氨酸,Nona-L-arginine; Peptide R9
Canonical SMILES [R][R][R][R][R][R][R][R][R]
分子式 C54H110N36O10 分子量 1423.69
溶解度 Soluble in DMSO 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 0.7024 mL 3.512 mL 7.024 mL
5 mM 0.1405 mL 0.7024 mL 1.4048 mL
10 mM 0.0702 mL 0.3512 mL 0.7024 mL
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