Cyclolinopeptide B
目录号 : GA21349
Cyclolinopeptide B是一种具有显著生物活性的环状九肽。
Cas No.:193139-41-2
Sample solution is provided at 25 µL, 10mM.
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Cyclolinopeptide B is a cyclic nonapeptide with significant biological activity [1]. Cyclolinopeptide B inhibits the activation of T cells and B cells and downregulates the expression of immune-related genes (such as IL-2 and IFN-γ) [2]. Cyclolinopeptide B is commonly used to treat cancer [3].
In Sk-Br-3 cells, Cyclolinopeptide B (25, 50, 100, 200, 400μg/mL; 24h, 48h) has concentration dependent cytotoxicity [4]. In human gastric SGC7901 cells, Cyclolinopeptide B (40, 80, 120, 160, 200, 240μM; 24h, 48h) induces apoptosis by activating Fas, FasL, caspase 8, and caspase 3 [5]. In human gastric SGC7901 cells, Cyclolinopeptide B (80, 120, 200μM; 24h) isolated from flaxseed induce G1 cell cycle arrest on SGC-7901 cells by modulating the AKT/JNK signaling pathway [6].
References:
[1]. Morita H, Shishido A, Matsumoto T, et al. A new immunosuppressive cyclic nonapeptide, cyclolinopeptide B from Linum usitatissimum. Bioorganic & Medicinal Chemistry Letters. 1997 May 20; 7(10): 1269-1272.
[2]. Aladedunye F, Sosinska E, Przybylski R. Flaxseed cyclolinopeptides: analysis and storage stability. Journal of the American Oil Chemists' Society. 2013 Mar; 90: 419-428.
[3]. Fojnica A, Gromilic Z, Vranic S, et al. Anticancer potential of the cyclolinopeptides. Cancers. 2023 Jul 30; 15(15): 3874.
[4]. Okinyo-Owiti DP, Dong Q, Ling B, et al. Evaluating the cytotoxicity of flaxseed orbitides for potential cancer treatment. Toxicology reports. 2015 Jan 1; 2: 1014-1018.
[5]. Zou XG, Hu JN, Li J, et al. iCellular uptake of [1–9-NαC]-linusorb B2 and [1–9-NαC]-linusorb B3 isolated from flaxseed, and their antitumor activities in human gastric SGC-7901 cells. Journal of Functional Foods. 2018 Sep 1; 48: 692-703.
[6]. Zou XG, Hu JN, Wang M, et al. [1–9-NαC]-linusorb B2 and [1–9-NαC]-linusorb B3 isolated from flaxseed induce G1 cell cycle arrest on SGC-7901 cells by modulating the AKT/JNK signaling pathway. Journal of Functional Foods. 2019 Jan 1; 52: 332-339.
Cyclolinopeptide B是一种具有显著生物活性的环状九肽 [1]。Cyclolinopeptide B抑制T细胞和B细胞的活化，并下调免疫相关基因（如IL-2和IFN-γ）的表达 [2]。Cyclolinopeptide B常用于治疗癌症 [3]。
在Sk-Br-3细胞中，Cyclolinopeptide B（25、50、100、200、400μg/mL；24h、48h）具有浓度依赖性细胞毒性 [4]。在人胃癌SGC7901细胞中，Cyclolinopeptide B（40、80、120、160、200、240μM；24h、48h）通过激活Fas、FasL、caspase 8和caspase 3诱导细胞凋亡 [5]。在人胃SGC7901细胞中，从亚麻籽中分离的Cyclolinopeptide B（80、120、200μM；24h）通过调节AKT/JNK信号通路诱导SGC-7901细胞G1细胞周期停滞 [6]。
| Cell experiment [1]: | |
Cell lines | Sk-Br-3 cells |
Preparation Method | The cultured cells were inoculated in 96-well plates, with 10,000 cells per well for the Sk-Br-3 cell line. After the inoculated cells grew to 70-80% confluence, they were treated with LOB for 24 hours and 48 hours, respectively. For Sk-Br-3 cells, the Cyclolinopeptide B administration concentrations were 400μg/mL, 200, 100, 50, and 25μg/mL, respectively. |
Reaction Conditions | 25, 50, 100, 200, 400μg/mL; 24h, 48h |
Applications | Cyclolinopeptide B has concentration dependent cytotoxicity in Sk-Br-3 cells. |
[1]. Okinyo-Owiti DP, Dong Q, Ling B, et al. Evaluating the cytotoxicity of flaxseed orbitides for potential cancer treatment. Toxicology reports. 2015 Jan 1; 2: 1014-1018. | |
| Cas No. | 193139-41-2 | SDF | |
| 分子式 | C56H83N9O9S | 分子量 | 1058.4 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9448 mL | 4.7241 mL | 9.4482 mL |
| 5 mM | 0.189 mL | 0.9448 mL | 1.8896 mL |
| 10 mM | 0.0945 mL | 0.4724 mL | 0.9448 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet