Foli-Ciocalteu'S phenol reagent(2M)

Foli-Ciocalteu'S phenol reagent(2M) is a chemical reagent commonly used to determine protein concentration and total phenol content. It is mainly used in Lowry method protein quantification and the determination of total phenol content in plant extracts^{[1, 2]}. Foli-Ciocalteu’S phenol reagent can react with phenolic substances and specific amino acids in proteins (such as tyrosine and tryptophan) under alkaline conditions to generate a blue complex^[3]. Foli-Ciocalteu’S phenol reagent is sensitive to a variety of interfering substances, and the experimental conditions must be strictly controlled, such as the influence of interfering substances such as buffers, chelating agents, detergents and cyclic organic molecules. Foli-Ciocalteu’S phenol reagent can be used as a spray reagent in chromatography technology^[4].

References:
[1] Chaudhary G, Kumar M, Swami N, et al. Evaluation of Physicochemical and Sensory Properties of Multi‐Nutrient Fortified Flours[J]. Food Safety and Health, 2025.
[2] Kim H S, Cha E, Kim Y H, et al. Raffinose, a plant galactoside, inhibits Pseudomonas aeruginosa biofilm formation via binding to LecA and decreasing cellular cyclic diguanylate levels[J]. Scientific reports, 2016, 6(1): 25318.
[3] Gao J. Properties of soy protein isolate films fortified with underutilized quinoa stalks and leaves[D]. University of Guelph, 2014.
[4] Ikawa M, Dollard C A, Schaper T D. Reaction of Folin-Ciocalteau phenol reagent with purines, pyrimidines, and pteridines and its relationship to structure[J]. Journal of Agricultural and Food Chemistry, 1988, 36(2): 309-311.

Foli-Ciocalteu'S phenol reagent(2M)是一种常用于测定蛋白质浓度和总酚含量的化学试剂，主要应用于Lowry法蛋白质定量和植物提取物中总酚含量的测定^{[1, 2]}。Foli-Ciocalteu’S phenol reagent在碱性条件下能与酚类物质和蛋白质中的特定氨基酸（如酪氨酸、色氨酸）反应，生成蓝色复合物^[3]。Foli-Ciocalteu’S phenol reagent对多种干扰物质敏感，实验时需严格控制条件，例如缓冲液、螯合剂、去垢剂和环状有机分子等干扰物的影响。Foli-Ciocalteu’S phenol reagent能够用作色谱技术中的喷雾试剂^[4]。