Ferroprotoporphyrin
(Synonyms: 羟高铁血红素) 目录号 : GC49230
An iron-containing cofactor
Cas No.:14875-96-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
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Ferroprotoporphyrin is an iron-containing cofactor.1,2 It is involved in several biological processes such as the transport or storage of oxygen by hemoglobin or myoglobin, respectively, electron transfer by cytochrome b5, and oxidation of xenobiotics or endogenous substrates by cytochrome P450s (CYPs).
1.Shimizu, T., Lengalova, A., MartÍnek, V., et al.Heme: Emergent roles of heme in signal transduction, functional regulation and as catalytic centresChem. Soc. Rev.48(24)5624-5657(2019) 2.Schneider, S., Marles-Wright, J., Sharp, K.H., et al.Diversity and conservation of interactions for binding heme in b-type heme proteinsNat. Prod. Rep.24(3)621-630(2007)
| Cas No. | 14875-96-8 | SDF | |
| 别名 | 羟高铁血红素 | ||
| Canonical SMILES | [O-]C(CCC(C1=CC(C(CCC([O-])=O)=C2C)=[N](C2=C3)[Fe+2]4([N-]5C3=C6C)[N-]1C7=CC(C(C=C)=C8C)=[N]4C8=CC5=C6C=C)=C7C)=O.[H+].[H+] | ||
| 分子式 | C34H30FeN4O4·2H | 分子量 | 616.5 |
| 溶解度 | DMF: 1 mg/ml,DMSO: slightly soluble | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6221 mL | 8.1103 mL | 16.2206 mL |
| 5 mM | 0.3244 mL | 1.6221 mL | 3.2441 mL |
| 10 mM | 0.1622 mL | 0.811 mL | 1.6221 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Correlation of antimalarial activity of artemisinin derivatives with binding affinity with Ferroprotoporphyrin IX
J Med Chem 1997 Feb 28;40(5):633-8.PMID:9057849DOI:10.1021/jm960767v.
The antimalarial activity of a number of artemisinin derivatives, both newly synthesized and currently used as drugs, against Plasmodium falciparum in culture shows a correlation with their affinity of binding with Ferroprotoporphyrin IX, as measured from the spectral change of the latter. The new C-16-functionalized artemisinin derivatives were obtained through a novel one-pot synthesis of artemisitene (2) from naturally abundant artemisinin (1), followed by Michael addition with nucleophiles. The correlation points to the biological significance of the interaction of these derivatives with Ferroprotoporphyrin IX and may provide a basis for primary screening of peroxidic antimalarials of similar structures.
Novel ferrocenic artemisinin derivatives: synthesis, in vitro antimalarial activity and affinity of binding with Ferroprotoporphyrin IX
Bioorg Med Chem 2000 Dec;8(12):2739-45.PMID:11131165DOI:10.1016/s0968-0896(00)00206-6.
Following our search for novel compounds with high antimalarial activity, a series of artemisinin (QHS) derivatives containing a ferrocenic nucleus was prepared and tested in vitro against Plasmodium falciparum strains. Two new metallocenic derivatives (1 and 3) were found as potent as QHS. All compounds showed a capacity to bind with Ferroprotoporphyrin IX. A decrease in the Soret band absorbance of Ferroprotoporphyrin IX, resulting from the addition of different drugs concentrations, was shown. The association stoichiometry of compounds to Ferroprotoporphyrin IX appears to be 1:2 at equilibrium, with an intermediate 1:1 complexation. These results appear to strengthen the role of adducts between artemisinin derivatives and heme in generation of artemisinin radicals. Such interaction of artemisinin ferrocenyl derivatives with Ferroprotoporphyrin IX and its biological significance could form a basis in future drug development.
Haem in the gut. Part II. Faecal excretion of haem and haem-derived porphyrins and their detection
J Gastroenterol Hepatol 1990 Mar-Apr;5(2):194-203.PMID:2103398DOI:10.1111/j.1440-1746.1990.tb01824.x.
Only a fraction of haem (Ferroprotoporphyrin) finding its way into the gut lumen is absorbed; the major portion enters the colon. There, unabsorbed haem, together with any haem of haemoproteins shed directly into the colonic lumen as haemoglobin or other haemoproteins, are converted by bacteria to a range of haem-derived porphyrins (HDP) lacking iron. This conversion is a slow and incomplete process and the amount converted in this way depends on colonic transit rate, site of bleeding and amount of luminal haem. As a consequence, faeces contain variable proportions of haem and HDP. The guaiac and tetramethylbenzidine tests give a qualitative index of faecal blood; they depend on the pseudoperoxidase activity of intact haem and cannot detect HDP. These tests perform better for large bowel bleeding than for more proximal bleeding. The fluorimetric HemoQuant assay quantitates both haem and HDP; it performs well for both proximal and distal bleeding. Neither type of test can allow for intestinal absorption of haem or HDP. Quantitation of gastrointestinal bleeding derived from measurement of faecally excreted haem and HDP is, therefore, likely to underestimate haem delivered into the gut lumen. In a given clinical situation, the choice of a haem-dependent occult blood test must take into account the possibility of colonic conversion of haem to HDP and the possible value of quantitation as opposed to qualitative detection.