Ethyl 3-Aminobenzoate (methanesulfonate)
(Synonyms: 3-氨基苯甲酸乙酯甲基磺酸盐,Ethyl m-Aminobenzoate, NSC 93790, Tricaine methanesulfonate , MS-222) 目录号 : GC15824
A fish anesthetic
Cas No.:886-86-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Ethyl 3-Aminobenzoate can block the generation of action potentials via voltage-dependent Na+-channels.
Sodium channels play a critical role in physiology: they can rapidly transmit depolarizing impulses throughout cells and cell networks, therefore enabling co-ordination of higher processes. Sodium channels are also of special importance for the history of physiology.
In vitro: The major mode of action for ethyl 3-aminobenzoate was found to be nervous system suppression whereby the entrance of sodium into the nerve was inhibited, therefore limiting nerve membrane excitability. In addition, the nerve inhibition was facilitated by the lipid solubility of ethyl 3-aminobenzoate, which allowed it to move easily into the cell membrane to bind with sodium channels [1].
In vivo: Previous study evaluated the potency and dynamics of ethyl 3-aminobenzoate-induced effects on neuronal firing of sensory and motor nerves alongside a defined motor behavior in Xenopus laevis tadpoles. Electrophysiological recordings of extraocular motor discharge and nerve activity were measured before, during and after administration of drugs. Results showed that ethyl 3-aminobenzoate and benzocaine, but not pancuronium could cause a dose-dependent, reversible blockade of extraocular motor and sensory nerve activity. Such results indicated that ethyl 3-aminobenzoate was able to block the activity of both sensory and motor nerves compatible with the mechanistic action of effective anesthetics, suggesting that ethyl 3-aminobenzoate was effective as single-drug anesthetic for surgical interventions in anamniotes [2].
Clinical trial: So far, no clinical study has been conducted.
References:
[1] K. M. Carter, C. M. Woodley and R. S. Brown. A review of tricaine methanesulfonate for anesthesia of fish. Rev. Fish Biol. Fisheries 21, 51-59 (2011).
[2] C. Ramlochansingh, F. Branoner, B. P. Chagnaud, et al. Efficacy of tricaine methanesulfonate (MS-222) as an anesthetic agent for blocking sensory-motor responses in Xenopus laevis tadpoles. PLoS One 9(7), e101606 (2014).
| Cas No. | 886-86-2 | SDF | |
| 别名 | 3-氨基苯甲酸乙酯甲基磺酸盐,Ethyl m-Aminobenzoate, NSC 93790, Tricaine methanesulfonate , MS-222 | ||
| 化学名 | 3-amino-benzoic acid-ethyl ester, monomethanesulfonate | ||
| Canonical SMILES | O=C(OCC)C1=CC(N)=CC=C1.O=S(O)(C)=O | ||
| 分子式 | C9H11NO2 • CH4O3S | 分子量 | 261.3 |
| 溶解度 | ≥ 13.25mg/mL in DMSO | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.827 mL | 19.1351 mL | 38.2702 mL |
| 5 mM | 0.7654 mL | 3.827 mL | 7.654 mL |
| 10 mM | 0.3827 mL | 1.9135 mL | 3.827 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。