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Cyclophilin F Rat Bioactive

目录号 : GP21531

Cyclophilin-F Rat Recombinant Bioactive

Cyclophilin F Rat Bioactive Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥72,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Cyclophilin F Rat Recombinant produced in E.Coli is asingle, non-glycosylated polypeptide chain containing 200 amino acids (30-206 a.a)and having a molecular mass of 21.2Da.Cyclophilin F is fused to a23 amino acid His-tag at N-terminus & purified by proprietarychromatographic techniques.

Product Data

Purity Greaterthan 90.0% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile filtered colorless solution. Shipping Condition Shipped with Ice Packs.
Synonyms Peptidyl-prolyl cis-trans isomerase F; mitochondrial; PPIaseF; Cyclophilin D; CyP-D; CypD; Cyclophilin F; Rotamase F.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAENFRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGPNTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESFGSKSGKTSKK IVITDCGQLS.
Stability Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Biological Activity Specificactivity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme thatcleaves 1nmol of suc-AAFP-PNA per minute at 37C in TrisиCHCl pH 8.0 using chymotrypsin.
Formulation CyclophilinF protein solution (1mg/ml) containing Phosphate Buffered Saline (pH 7.4), 10%glycerol and 1mM DTT.

Introduction

PPIF is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIF accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Biological Activity

Specificactivity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme thatcleaves 1nmol of suc-AAFP-PNA per minute at 37C in TrisиCHCl pH 8.0 using chymotrypsin.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.