Cy5-UTP, 10mM Sodium Solution
目录号 : GB20007
Cy5-UTP是Cyanine5标记的UTP类似物,可以在体外转录反应中替代UTP进行RNA标记。
Sample solution is provided at 25 µL, 10mM.
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Cyanine5-UTP (Cy5-UTP) can replace UTP as the substrate of T7 RNA polymerase, and generate orange fluorescently labeled mRNA through in vitro transcription, excitation/emission (nm): 650/665nm[1]. Probes generated using Cy5-UTP as a substrate are suitable for multicolor fluorescence analysis, especially dual-color expression arrays combined with Cy5-UTP [2].
References:
[1]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017 Jul;26(7):1363-1379.
[2]. Guerra (2006) Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques 41 (1):53.
Cyanine5-UTP (Cy5-UTP)可替代UTP作为T7 RNA聚合酶的底物,通过体外转录生成带有橙色荧光标记的mRNA,激发/发射(nm):650/665nm [1]。使用Cy5-UTP作为底物产生的探针适用于多色荧光分析,特别是与Cy5-UTP结合的双色表达阵列[2]。
| Cas No. | SDF | ||
| 化学名 | O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([C@H]4[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O | ||
| 分子式 | C45H58N5O22P3S2 | 分子量 | 1178.01 |
| 溶解度 | A solution in water | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.8489 mL | 4.2444 mL | 8.4889 mL |
| 5 mM | 0.1698 mL | 0.8489 mL | 1.6978 mL |
| 10 mM | 0.0849 mL | 0.4244 mL | 0.8489 mL |
Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase
Biotechniques2006 Jul;41(1):53-6.PMID: 16869513DOI: 10.2144/000112182
A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single fluorophore can be covalently attached at the 3' end of each oligonucleotide probe molecule in the spot. Fluorescein-12-ddUTP CyTM3-ddUTP Cy5-UTP, and Cy3-UTP were compared as TdT substrates for 3' end labeling an array of 1273 hexamer probes. Cy5-UTP was found to show minimal bias toward probe base composition and is therefore well suited for quantitative analysis of microarray spots where the oligonucleotide probes are coupled via a 5' end linkage to the solid phase.