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CSTA Human, Active

目录号 : GP23264

Cystatin-A Human Recombinant, Active

CSTA Human, Active Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
20μg
¥2,030.00
5-10工作日
1mg
¥50,400.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Cystatin A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 118 amino acids (1-98a.a.) and having a molecular mass of 13.1 kDa.The Cystatin A is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95% as determined by reducing SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered clear solution. Shipping Condition Shipped with Ice Packs.
Synonyms Cystatin-A; Cystatin-AS; Stefin-A; CSTA; STF1; STFA.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Biological Activity The IC50 value is < 1.0nM. The inhibitory function of CSTA on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
Formulation Cystatin-A (1mg/ml) in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

Introduction

Human Cystatin A (CSTA or Stefin A) belongs to family 1 of the cystatin superfamily, which is characterized by the lack of disulphide bonds and carbohydrates. CSTA is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as Cathepsins B, H and L. Cystatin A has also been implicated in several disease states. Because of altered proteolytic state in cancer progression, CSTA may have a role in the proteolitic pathways.

Biological Activity

The IC50 value is < 1.0nM. The inhibitory function of CSTA on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.