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CKB Human, Pichia

目录号 : GP22461

Creatine Kinase Brain Human Recombinant, Pichia

CKB Human, Pichia Chemical Structure

规格 价格 库存 购买数量
10μg
¥840.00
5-10工作日
50μg
¥2,030.00
5-10工作日
1mg
¥13,230.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

CKBB Human Recombinant produced in Pichia Pastoris is a dimeric glycosylated full length polypeptide chain comprised of 2 identical B subunits and having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and having a Mw of 47kDaThe CKBB is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Source Pichia Pastoris.
Phycical Appearance Sterile Filtered colourless liquid formulation. Shipping Condition Shipped with Ice Packs.
Synonyms Creatine kinase B-type; EC 2.7.3.2; Creatine kinase B chain; B-CK; CKB; CKBB; CKBBI.
Stability CKBB should be stored below -18°C . Please prevent freeze-thaw cycles.
Biological Activity The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 ?mole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.
Formulation CKBB Human contains 10Mm Bis-Tris-HCl pH-6.0, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.

Introduction

Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Biological Activity

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 ?mole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.

Stability

CKBB should be stored below -18°C . Please prevent freeze-thaw cycles.