Chrysin
(Synonyms: 白杨素; 5,7-Dihydroxyflavone) 目录号 : GN10028
Chrysin是一种天然的黄酮，存在于多种植物提取物、蜂蜜和蜂胶中，具有多种生物活性，包括抗炎、抗氧化、抗癌等作用。
Cas No.:480-40-0
Sample solution is provided at 25 µL, 10mM.
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Chrysin is a natural flavonoid found in various plant extracts, honey, and propolis, possessing diverse biological activities, including anti-inflammatory, antioxidant, and anticancer effects[1, 2]. Chrysin can regulate drug resistance by inhibiting P-glycoprotein activity, thereby increasing the intracellular concentration of chemotherapeutic drugs such as paclitaxel[3].
In vitro, treatment of DU145 and PC-3 cells with Chrysin (0-100µM) for 48h induced apoptosis, evidenced by DNA fragmentation and an increase in the number of cells in the sub-G1 phase of the cell cycle in both DU145 and PC-3 cells[4]. Treatment of U937 cells with Chrysin (5-10µM) for 12h induced apoptosis and significantly induced the release of cytochrome c from mitochondria into the cytoplasm[5]. Treatment of human melanoma cells (SP6.5 and M17 cells) with Chrysin (0-100µM) for 12h reduced cell viability in a dose-dependent manner, with IC50 values of 28.3µM and 35.8µM for SP6.5 and M17 cells, respectively[6].
In vivo, oral administration of Chrysin (5, 10mg/kg) to mice with colitis for 7 days significantly improved the damage to the colonic tissue structure, reduced colonic myeloperoxidase (MPO) activity, and decreased the production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines[7].
References:
[1] Zeinali M, Rezaee S A, Hosseinzadeh H. An overview on immunoregulatory and anti-inflammatory properties of chrysin and flavonoids substances[J]. Biomedicine & Pharmacotherapy, 2017, 92: 998-1009.
[2] Stompor-Gorący M, Bajek-Bil A, Machaczka M. Chrysin: Perspectives on contemporary status and future possibilities as pro-health agent[J]. Nutrients, 2021, 13(6): 2038.
[3] Panneerselvam S, Hussain S, Jeyavelkumaran R. Chrysin as a Chemosensitizer: Molecular Insights into Its Role in Prostate Cancer Treatment[J]. Journal of Bio-X Research, 2025, 2025: 0060.
[4] Ryu S, Lim W, Bazer F W, et al. Chrysin induces death of prostate cancer cells by inducing ROS and ER stress[J]. Journal of cellular physiology, 2017, 232(12): 3786-3797.
[5] Woo K J, Jeong Y J, Park J W, et al. Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells[J]. Biochemical and biophysical research communications, 2004, 325(4): 1215-1222.
[6] Xue C, Chen Y, Hu D N, et al. Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis[J]. Oncology Letters, 2016, 12(6): 4813-4820.
[7] Shin E K, Kwon H S, Kim Y H, et al. Chrysin, a natural flavone, improves murine inflammatory bowel diseases[J]. Biochemical and biophysical research communications, 2009, 381(4): 502-507.
Chrysin是一种天然的黄酮，存在于多种植物提取物、蜂蜜和蜂胶中，具有多种生物活性，包括抗炎、抗氧化、抗癌等作用[1, 2]。Chrysin能够通过抑制P糖蛋白活性调节药物耐药性，从而提高如紫杉醇等化疗药物的细胞内浓度[3]。
在体外，Chrysin（0-100µM）处理DU145和PC-3细胞48h，诱导了细胞凋亡，表现为DNA片段化，并增加了DU145和PC-3细胞在细胞周期的亚G1期细胞数量[4]。Chrysin（5-10µM）处理U937细胞12h，诱导了细胞凋亡，显著诱导细胞色素c从线粒体释放到细胞质中[5]。Chrysin（0-100µM）处理人黑色素瘤细胞（SP6.5和M17细胞）12h，以剂量依赖性方式降低了细胞的活力，对SP6.5和M17细胞的IC50值分别为28.3µM和35.8µM[6]。
在体内，Chrysin（5, 10mg/kg）通过口服治疗结肠炎小鼠7天，显著改善了小鼠的结肠组织结构的破坏，降低了结肠髓过氧化物酶（MPO）活性，减少了一氧化氮（NO）、前列腺素E2（PGE2）和促炎细胞因子等炎症介质的产生[7]。
| Cell experiment [1]: | |
Cell lines | DU145、PC-3 cells |
Preparation Method | DU145 and PC-3 cells (2×105 cells) were seeded on six-well plates and incubated for 24h in serum-free medium until cells reached 70–80% confluency. Then, cells were treated with 0, 5, 10, 20, 50, 100μM Chrysin for 48h at 37°C in a CO2 incubator. Supernatants were transferred from culture dishes into collecting tubes and adherent cells detached with trypsin-EDTA. The cells were collected by centrifugation, washed twice with cold PBS, and fixed with chilled 70% ethanol at 4°C overnight. Cells were then washed twice with PBS, incubated with 10mg/mL RNase A and 50mg/mL propidium iodide (PI) in PBS at room temperature for 30min in the dark and subjected to flow cytometry using a FACSCalibur. |
Reaction Conditions | 0, 5, 10, 20, 50, 100μM; 48h |
Applications | Percentages of DU145 and PC-3 cells in the sub-G1 phase of the cell cycle increased with increasing concentrations of Chrysin. |
| Animal experiment [2]: | |
Animal models | BALB/c mice |
Preparation Method | To induce experimental colitis, we treated the mice for 7 days with 5% DSS dissolved in filter-purified water. The control mice received filtered water alone. Chrysin was dissolved in dimethyl sulfoxide (DMSO) and was freshly diluted in corn oil. Chrysin (1, 5, or 10mg/kg of body weight) or the vehicle (PBS with DMSO) was administrated orally for 7days, beginning in coordination with the start of dextran sodium sulfate (DSS) exposure. Assessment of DSS-induced colitis. The mice were assessed daily for the development of colitis based on body weight, gross rectal bleeding, stool consistency, and survival. Overall disease severity was assessed using a clinical scoring system with a scale of 0-4. |
Dosage form | 1, 5, 10mg/kg; 7 days; p.o. |
Applications | A significant blunting of weight loss and clinical signs was observed in DSS-exposed, Chrysin-treated mice when compared to vehicle-treated mice. This was associated with a remarkable amelioration of the disruption of the colonic architecture, a significant reduction in colonic myeloperoxidase (MPO) activity, and a decrease in the production of inflammatory mediators such as nitric oxide (NO), prostaglandin (PG) E2, and pro-inflammatory cytokines. |
References: | |
| Cas No. | 480-40-0 | SDF | |
| 别名 | 白杨素; 5,7-Dihydroxyflavone | ||
| 化学名 | 5,7-dihydroxy-2-phenylchromen-4-one | ||
| Canonical SMILES | C1=CC=C(C=C1)C2=CC(=O)C3=C(C=C(C=C3O2)O)O | ||
| 分子式 | C15H10O4 | 分子量 | 254.24 |
| 溶解度 | ≥ 12.4mg/mL in DMSO | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9333 mL | 19.6665 mL | 39.3329 mL |
| 5 mM | 786.7 μL | 3.9333 mL | 7.8666 mL |
| 10 mM | 393.3 μL | 1.9666 mL | 3.9333 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.50%
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