Cholesterol-d6
(Synonyms: 胆固醇-26,26,26,27,27,27-D6) 目录号 : GC40203An internal standard for the quantification of cholesterol
Cas No.:60816-17-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cholesterol-d6 is intended for use as an internal standard for the quantification of cholesterol by GC- or LC-MS. Cholesterol is a major sterol produced in mammalian cells that is required for cell viability and proliferation.[1] It is a component of mammalian cell membranes that interacts with membrane phospholipids, sphingolipids, and proteins to influence their behavior. Cholesterol is a precursor of steroid hormones, bile acids, and the active form of vitamin D. Impaired cholesterol homeostasis is related to development of various diseases including fatty liver, diabetes, gallstones, dyslipidemia, atherosclerosis, heart attack, and stroke.[2]
Reference:
[1]. Ohvo-Rekilä, H., Ramstedt, B., Leppimäki, P., et al. Cholesterol interactions with phospholipids in membranes. Prog. Lipid Res. 41(1), 66-97 (2002).
[2]. Yamanashi, Y., Takada, T., and Suzuki, H. Associations between lifestyle-related diseases and transporters involved in intestinal absorption and biliary excretion of cholesterol. Biol. Pharm. Bull. 41(1), 1-10 (2018).
| Cas No. | 60816-17-3 | SDF | |
| 别名 | 胆固醇-26,26,26,27,27,27-D6 | ||
| 化学名 | cholest-5-en-26,26,26,27,27,27-d6-3β-ol | ||
| Canonical SMILES | C[C@H](CCCC(C([2H])([2H])[2H])C([2H])([2H])[2H])[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C | ||
| 分子式 | C27H40D6O | 分子量 | 392.7 |
| 溶解度 | Slightly soluble in chloroform | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5465 mL | 12.7324 mL | 25.4647 mL |
| 5 mM | 0.5093 mL | 2.5465 mL | 5.0929 mL |
| 10 mM | 0.2546 mL | 1.2732 mL | 2.5465 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Microwave assisted direct saponification for the simultaneous determination of cholesterol and cholesterol oxides in shrimp
J Steroid Biochem Mol Biol 2017 May;169:88-95.PMID:27013019DOI:10.1016/j.jsbmb.2016.03.027
A novel microwave-assisted direct saponification method for the simultaneous determination of cholesterol and cholesterol oxides in shrimp was developed and validated. Optimal saponification conditions, determined by means of an experimental design, were achieved using 500mg of sample and 20mL of 1mol/L KOH ethanol solution for 16min at 45°C at maximum power at 200W and magnetic stirring at 120rpm. Higher extraction of cholesterol oxides in a reduced saponification time (∼75 times) was achieved in comparison with the direct cold saponification method. The new method showed low detection (≤0.57μg/mL) and quantification (≤1.73μg/mL) limits, good repeatability (≤10.50% intraday and ≤8.56% interday) and low artifact formation (evaluated by using a deuterated Cholesterol-d6 standard). Raw, salted and dried-salted shrimps were successfully analyzed by the validated method. The content of cholesterol oxides increased after salting and decreased after drying.