Cholesterol-13C3
(Synonyms: 胆固醇 13C3) 目录号 : GC63843
Cholesterol-13C3 是一种 13C 标记的 Cholesterol。Cholesterol 是一种哺乳动物中的主要固醇，占质膜结构成分的 20-25％。 质膜对水具有高渗透性，但对离子和质子则相对不可渗透。Cholesterol 在确定膜的流动性和渗透性特征以及转运蛋白和信号蛋白的功能中起着重要作用。Cholesterol 还是一种内源性雌激素相关受体 α (ERRα) 激动剂。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cholesterol-13C3 is the 13C-labeled Cholesterol. Cholesterol is the major sterol in mammals and is makes up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins[1][2]. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist[3].
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216.
[2]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14.
[3]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14.
[4]. Dietschy JM, et al. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J Lipid Res. 2004 Aug;45(8):1375-97.
[5]. Dietschy JM, et al. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J Lipid Res. 2004 Aug;45(8):1375-97.
[6]. Casaburi I, et al. Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment. Front Endocrinol (Lausanne). 2018 Sep 11;9:525.
[7]. Casaburi I, et al. Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment. Front Endocrinol (Lausanne). 2018 Sep 11;9:525.
| Cas No. | SDF | Download SDF | |
| 别名 | 胆固醇 13C3 | ||
| 分子式 | C2413C3H41O | 分子量 | 384.59 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6002 mL | 13.0009 mL | 26.0017 mL |
| 5 mM | 0.52 mL | 2.6002 mL | 5.2003 mL |
| 10 mM | 0.26 mL | 1.3001 mL | 2.6002 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Determination of serum cholesterol by a modification of the isotope dilution mass spectrometric definitive method
Anal Chem 1989 Aug 1;61(15):1718-23.PMID:2672877DOI:10.1021/ac00190a025.
An isotope dilution mass spectrometric (ID/MS) method for cholesterol is described that uses capillary gas chromatography with Cholesterol-13C3 as the labeled internal standard. Labeled and unlabeled cholesterol are converted to the trimethylsilyl ether. Combined capillary column gas chromatography and electron impact mass spectrometry are used to obtain the abundance ratio of the unlabeled and labeled [M+.] ions from the derivative. Quantitation is achieved by measurement of each sample between measurements of two standards whose unlabeled/labeled ratios bracket that of the sample. Seven pools were analyzed by this method: standard reference material (SRM) 1951, which consists of three frozen serum pools with low, medium, and high levels of cholesterol; SRM 1952, which consists of three freeze-dried serum pools with low, medium, and high levels of cholesterol; and SRM 909, a freeze-dried serum pool. The method is a modification of our original definitive method for cholesterol. The modified method uses much better chromatographic separations to assure specificity and a new method of implementing selected ion monitoring on a magnetic mass spectrometer to obtain high-precision measurements of ion intensity ratios on narrow gas chromatographic peaks. The modified method has a coefficient of variation (CV) of 0.22%, which is an improvement over the original method's CV of 0.36%. The measurements were found to be free of interference. The high precision and absence of bias qualify this method as a candidate definitive method.
The how and why of an accuracy base for proficiency testing programs
Arch Pathol Lab Med 1988 Apr;112(4):343-5.PMID:3355329doi
The National Bureau of Standards (NBS) has been involved for more than ten years in development of definitive methods for measuring important inorganic and organic analytes in human serum, including calcium, potassium, chloride, cholesterol, glucose, urea, uric acid, and creatinine. These methods are carefully designed and thoroughly tested isotope dilution mass spectrometric procedures that produce results of high precision and accuracy. Definitive methods are used to certify concentrations in reference serum pools, which are distributed among clinical laboratories as a means of assessing the accuracy of the routine methods. The College of American Pathologists operates a major proficiency testing program for clinical laboratories. The National Bureau of Standards works with the College of American Pathologists to assign values for analyte concentrations in proficiency testing samples where possible, and these values are used to assess the overall accuracy of the participants (grand mean) as well as the accuracy of peer groups (those that use the same methodology and/or instrumentation). For the analytes studied to date, the grand mean values have generally been close to the definitive values, but some of the peer group results have shown significant biases. To illustrate the definitive method development process, a modified and improved definitive method for cholesterol measurement is described. The modified method uses a Cholesterol-13C3 as the labeled internal standard, capillary gas chromatography for sample introduction, and a novel means of electrically switching between the ions being measured. Results on standard reference material 909, a lyophilized human serum, demonstrate the precision and absence of significant measurement bias attainable with the new method.