Cacodylic Acid (sodium salt hydrate)
(Synonyms: 二甲砷酸钠三水合物) 目录号 : GC47018
A reagent used in electron microscopy techniques
Cas No.:6131-99-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cacodylic acid has been widely used in buffers for electron microscopy techniques.1 It has also been used to introduce arsenic into proteins for single-wavelength anomalous diffraction (SAD) phasing in protein crystallography.2 Cacodylic acid has also been used as an herbicide, acting as a defoliant.3 In rats exposed to carcinogens, cacodylic acid increases the likelihood of tumor formation in the bladder, kidney, liver, and thyroid gland at concentrations as low as 50 ppm for the bladder.4
1.Graham, L., and Orenstein, J.M.Processing tissue and cells for transmission electron microscopy in diagnostic pathology and researchNat. Protoc.2(10)2439-2450(2007) 2.Liu, X., Zhang, H., Wang, X.J., et al.Get phases from arsenic anomalous scattering: De novo SAD phasing of two protein structures crystallized in cacodylate bufferPLoS One6(9)1-8(2011) 3.Mastradone, P.J., and Woolson, E.A.Levels of arsenical species in cotton after field application of a cacodylic acid defoliantBull. Environ. Contam. Toxicol.31(2)216-221(1983) 4.Yamamoto, S., Konishi, Y., Matsuda, T., et al.Cancer induction by an organic arsenic compound, dimethylarsinic acid (cacodylic acid), in F344/DuCrj rats after pretreatment with five carcinogensCancer Res.55(6)1271-1276(1995)
| Cas No. | 6131-99-3 | SDF | |
| 别名 | 二甲砷酸钠三水合物 | ||
| Canonical SMILES | C[As]([O-])(C)=O.[Na+].O.O.O | ||
| 分子式 | C2H6AsO2.Na [3H2O] | 分子量 | 214 |
| 溶解度 | Ethanol: 400 mg/mL,Water: 100 g/L | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.6729 mL | 23.3645 mL | 46.729 mL |
| 5 mM | 0.9346 mL | 4.6729 mL | 9.3458 mL |
| 10 mM | 0.4673 mL | 2.3364 mL | 4.6729 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A modified method of fine-granular cationic iron colloid preparation: its use in light and electron microscopic detection of anionic sites in the rat kidney glomerulus and certain other tissues
Arch Histol Jpn 1986 Mar;49(1):13-23.PMID:3089191DOI:10.1679/aohc.49.13
Ferric chloride, when boiled with hydrazine hydrate and Cacodylic Acid, is converted into a fine cationic iron (ferric hydroxide) colloid which consists of 0.5-1.5 nm electron-dense granules, and gives a distinct Prussian blue reaction. This colloid allows light and electron microscopic detection of ionized anionic sites in tissues at a wide pH range of 0.8-7.6. It is smaller in size and more stable, and assures longer and greater staining of tissues, especially at low pH levels, than the iron colloid prepared with sodium or ammonium cacodylate by Seno and his associates (1983, 1984, 1985). Some light and electron micrographs of the rat kidney, spleen and other organs stained with our colloid are presented as examples. These micrographs confirm that the glomerular podocyte end-foot surface facing the Bowman's capsular space is strongly negative-charged. They also show that almost all lymphoid cells around the arteries in the splenic white pulp and thymic cortex contain strongly negative-charged nuclei and that the distal convoluted and collecting urinary tubules are more negative-charged than the proximal convoluted tubules.