C17 Ceramide (d18:1/17:0)
(Synonyms: Cer(d18:1/17:0), Ceramide (d18:1/17:0), N-heptadecanoyl-D-erythro-Sphingosine) 目录号 : GC43038C17 Ceramide (d18:1/17:0)是一种合成神经酰胺，含有酰化为鞘氨醇的非天然 C17:0脂肪酸。
Cas No.:67492-16-4
Sample solution is provided at 25 µL, 10mM.
C17 Ceramide (d18:1/17:0) is a synthetic ceramide containing a non-natural C17:0 fatty acid acylated to sphingosine[1]. Ceramide regulates many different biological activities, such as apoptosis, cell differentiation, smooth muscle cell proliferation, and inhibition of the mitochondrial respiratory chain[2, 3]. C17 Ceramide (d18:1/17:0) can be used as an internal standard or reference for mass spectrometry (LC-MS/MS) or chromatography (HPLC) analysis of natural ceramides in biological samples[4, 5]. C17 Ceramide (d18:1/17:0) can be used as a potential biomarker to distinguish metabolically healthy obesity (MHO) from metabolically unhealthy obesity (MUO)[6].
References:
[1] Huang Q, Hao S, Yao X, et al. Quantitative LCMS for ceramides/dihydroceramides: Pregnancy baseline biomarkers and potential metabolic messengers[J]. bioRxiv, 2020: 2020.02. 24.963462.
[2] Birbes H, El Bawab S, Obeid L M, et al. Mitochondria and ceramide: intertwined roles in regulation of apoptosis[J]. Advances in enzyme regulation, 2002, 42: 113-129.
[3] Kogot-Levin A, Saada A. Ceramide and the mitochondrial respiratory chain[J]. Biochimie, 2014, 100: 88-94.
[4] Kasumov T, Huang H, Chung Y M, et al. Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry[J]. Analytical Biochemistry, 2010, 401(1): 154-161.
[5] Qiu L P, Sun W J. Simultaneous quantitative analysis of different ceramide species in cells by high-performance liquid chromatography-tandem mass spectrometry[J]. Zhejiang da xue xue bao. Yi xue ban= Journal of Zhejiang University. Medical Sciences, 2015, 44(4): 429-434.
[6] Yu B, Hu M, Jiang W, et al. Ceramide d18: 1/24: 1 as a potential biomarker to differentiate obesity subtypes with unfavorable health outcomes[J]. Lipids in Health and Disease, 2023, 22(1): 166.
C17 Ceramide (d18:1/17:0)是一种合成神经酰胺，含有酰化为鞘氨醇的非天然 C17:0脂肪酸[1]。神经酰胺调节许多不同的生物活动，例如细胞凋亡、细胞分化、平滑肌细胞增殖和线粒体呼吸链的抑制等[2, 3]。C17 Ceramide (d18:1/17:0)能够作为内标（internal standard）或参照物，用于质谱（LC-MS/MS）或色谱（HPLC）分析生物样本中的天然神经酰胺[4, 5]。C17 Ceramide (d18:1/17:0)能够作为一种潜在的生物标志物来区分代谢健康肥胖（MHO）和代谢不健康肥胖（MUO）[6]。
| Cas No. | 67492-16-4 | SDF | |
| 别名 | Cer(d18:1/17:0), Ceramide (d18:1/17:0), N-heptadecanoyl-D-erythro-Sphingosine | ||
| Canonical SMILES | OC[C@H](NC(CCCCCCCCCCCCCCCC)=O)[C@H](O)/C=C/CCCCCCCCCCCCC | ||
| 分子式 | C35H69NO3 | 分子量 | 551.9 |
| 溶解度 | Chloroform: Soluble,Ethanol: Heated,Methanol: Heated | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8119 mL | 9.0596 mL | 18.1192 mL |
| 5 mM | 0.3624 mL | 1.8119 mL | 3.6238 mL |
| 10 mM | 0.1812 mL | 0.906 mL | 1.8119 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet