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Bradford Protein Quantification Kit

目录号 : GK10027

Bradford Protein Quantification Kit是一种能够快速测定蛋白浓度的试剂盒,检测范围在0.025-2mg/ml。

Bradford Protein Quantification Kit Chemical Structure

规格 价格 库存 购买数量
125mL * 2 (1000T)
¥150.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

实验参考方法

1、标准曲线的绘制

  1.1用0.9%NaCl、PBS或水稀释蛋白标准品。蛋白标准(2mg/ml BSA)如果冻存,请完全融化并混匀后使用。

1.2 按照下表配制新的标准品体系。每次稀释时注意充分混匀。

编号

稀释液体积(μl)

标准品体积(μl)

最终浓度(μg/ml)

A

0

200 μl of 2 mg/ml Stock

2000 μg/ml

B

30 μl

90 μl of tube A

1500 μg/ml

C

60 μl

60 μl of tube A

1000 μg/ml

D

60 μl

200 μl of tube B

750 μg/ml

E

60 μl

300 μl of tube C

500 μg/ml

F

60 μl

300 μl of tube E

250 μg/ml

G

60 μl

300 μl of tube F

125 μg/ml

H

100 μl

100 μl of tube G

25 μg/ml

I

60 μl

0

0 (blank)

 

2、取5μl不同浓度蛋白标准品加到96孔板的蛋白标准孔中。

3、取5μl蛋白样品到96孔板的样品孔中。

注意:

①     如果样品不足5μl,需加标准品稀释液补足到5μl;

② 请注意记录样品体积。

4、每孔加入250μl Bradford染色液。

5、用酶标仪测定595nm处的吸光度,或560-610nm之间的其它波长的吸光度。

注意:可以立即测定吸光度,也可以在2小时内测定,2小时内检测数据无显著变化。

6、根据标准曲线和使用的样品体积计算出样品中的蛋白浓度。

产品描述

Bradford Protein Quantification Kit is a protein concentration determination kit with fast detection speed, good stability, high sensitivity, and the detection data has a good linear relationship.

This kit is developed based on the Bradford method, one of the two most commonly used protein concentration detection methods. It is one of the most sensitive protein concentration detection methods currently commonly used. After the Coomassie Brilliant Blue G-250 dye in the dye solution binds to the protein, the maximum absorption peak of the dye changes from A456 to A595, and the measured absorbance value is proportional to the protein concentration. Therefore, the protein concentration in the solution can be determined by detecting the absorbance at 595 nm, achieving a quick and easy protein concentration determination.

The detection speed of this kit is extremely fast. It takes less than 10 minutes to process 10-20 samples. Compared with the BCA method, the detection time is greatly shortened. It is not affected by the chemical reagents in most samples, and the detection results are reliable.

Bradford Protein Quantification Kit是蛋白浓度测定试剂盒,检测速度快,稳定性好,灵敏度高,并且检测数据有很好的线性关系。

本试剂盒是基于最常用的两种蛋白浓度检测方法之一Bradford法研制而成,是目前常用的灵敏度较高的蛋白浓度测定方法之一。染液中的考马斯亮蓝G-250染料与蛋白结合后,使染料的最大吸收峰从A456变为A595,且测定的吸光值与蛋白浓度成正比关系。因此,可通过检测595nm处的吸光度对溶液中蛋白质浓度进行测定,实现了快速简便的蛋白浓度测定。

本试剂盒检测速度极快,10-20个样品耗时不足10分钟,和BCA法相比大大缩短了检测时间,且不受大多数样品中化学试剂的影响,检测结果可靠。

产品组成

Components 1000 tests
Bradford 蛋白染色液 125mL * 2
BSA蛋白标准品(2000μg/ml ) 1mL * 5

功能属性

应用&特点蛋白质印迹
蛋白质表达测定
蛋白质分析和表征
蛋白质定量测定
运输方式蓝冰运输.
储存条件Bradford 蛋白染色液4°C保存, 一年有效。蛋白标准室温保存, 一个月有效; 4°C保存, 一年有效; -20°C可以长期保存。
用途仅供研究使用!不能用于人体。