Bovine Serum Albumin(Fatty Acid & lgG Free)

BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments. BSA protein is a single polypeptide chain consisting of ~583 amino acid residues and no carbohydrates and is approximately 66kDa in size. Fraction V refers to the fifth fraction of the original Cohn purification of plasma proteins using cold ethanol precipitation and is still commonly used as a name for bovine serum albumin regardless of purification method. Fatty Acid & IgG Free BSA allows for better formulation of albumin with specific fatty acids and IgG to enhance your cell culture system performance or reduce lipid & IgG interference with specific kits or assays. Fatty Acid Free BSA is ideally suited for fatty acid sensitive cell culture systems, such as CHO, Vero and MDCK cells, as well as for the most sensitive research applications. This BSA is protease free and certified BVD (Bovine Viral Diarrhoea) free. The presence of IgG can alter important experimental data and lead to additional non-specific binding in important immunoassays. In monoclonal antibody production the presence of IgG can result in reduced purification yields of the target protein. This BSA is IgG free.

BSA是一种高度纯化的冻干白蛋白，适用于蛋白质、酶系统、蛋白质标准品、杂交和蛋白酶敏感免疫分析。它还非常适合作为细胞培养基中的营养物，具有低干扰和低背景，是大多数标准分子生物学实验的理想选择。BSA蛋白是由约583个氨基酸残基组成的单一多肽链，不含碳水化合物，大小约为66kDa。级分V指的是使用冷乙醇沉淀对血浆蛋白进行初始Cohn纯化的第五级分，并且无论使用何种纯化方法，它仍被普遍用作牛血清白蛋白的名称。不含脂肪酸和IgG的BSA可以更好地配制含特定脂肪酸和IgG的白蛋白，从而提高细胞培养系统的性能或减少脂质和IgG对特定试剂盒或检测的干扰。不含脂肪酸的牛血清白蛋白非常适合脂肪酸敏感细胞培养系统，如CHO、Vero和MDCK细胞，以及最敏感的研究应用。这种牛血清白蛋白不含蛋白酶，经认证不含BVD（牛病毒性腹泻）。IgG的存在会改变重要的实验数据，并在重要的免疫分析中导致额外的非特异性结合。在单克隆抗体生产中，IgG的存在会导致目标蛋白的纯化产率降低。这种牛血清白蛋白不含IgG。