Bovine Serum Albumin(Fatty Acid & lgG Free)
(Synonyms: BSA，牛血清白蛋白来源于牛血清,牛血清白蛋白,白蛋白牛血清) 目录号 : GC26270Bovine Serum Albumin(Fatty Acid & lgG Free)是一种由583个氨基酸残基组成的球状蛋白质和寡核苷酸结合蛋白，由三个同源的全α结构域组成。
Cas No.:9048-46-8
Sample solution is provided at 25 µL, 10mM.
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Bovine Serum Albumin(Fatty Acid & lgG Free) is a globular protein and oligonucleotide-binding protein composed of 583 amino acid residues and consisting of three identical α-helical domains [1]. Bovine Serum Albumin can inhibit the overall binding of oligonucleotides to cells [2]. Bovine Serum Albumin is commonly used as a blocking agent in immunohistochemistry and is also applicable for proteins, enzyme systems, protein standards, hybridization, and proteinase-sensitive immunoassays [3-4].
In vitro, at pathological physiological glucose concentrations, Bovine Serum Albumin (0.373mM; 8, 16, and 24h) treatment significantly increased the LDH activity of human mature adipocytes and the content of carbonyl groups and the release of isoprostanes in the cells [5]. Bovine Serum Albumin (1mg/mL; 18h) pretreatment dose-dependently increased the NF-κB promoter activity and the gene expression of TNF-α in mouse peritoneal macrophages (RAW264.7z) stimulated by lipopolysaccharide (LPS) [6].
In vivo, a single dose of Bovine Serum Albumin (10mg/kg/day; i.p.) significantly attenuated the pro-inflammatory response in mice with endotoxin shock model and reduced the content of TNF-α in the mouse plasma [6]. Bovine Serum Albumin (0.1%, 250μl; twice weekly for 4 or 8 weeks; i.p.) treatment significantly increased the number of eosinophils in the adipose tissue of diet-induced obese mouse models and promoted the gene expression of type 2 cytokines and the generation of IgE [7].
References:
[1] Holm NK, Jespersen SK, Thomassen LV, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007;1774(9):1128-1138.
[2] Geselowitz DA, Neckers LM. Bovine serum albumin is a major oligonucleotide-binding protein found on the surface of cultured cells. Antisense Res Dev. 1995;5(3):213-217.
[3] Chwastowicz A, Wolny A, Sobień M, et al. Use of bovine serum albumin might impair immunofluorescence signal in thick tissue samples[J]. Scientific Reports, 2025, 15(1): 21920.
[4] Gómez C E, López-Campistrous A E, Duarte C A. An immunoassay with bovine serum albumin coupled peptides for the improved detection of anti V3 antibodies in HIV-1 positive human sera[J]. Journal of virological methods, 1998, 71(1): 7-16.
[5] Chesne S, Rondeau P, Armenta S, Bourdon E. Effects of oxidative modifications induced by the glycation of bovine serum albumin on its structure and on cultured adipose cells. Biochimie. 2006;88(10):1467-1477.
[6] Wheeler D S, Giuliano Jr J S, Lahni P M, et al. The immunomodulatory effects of albumin in vitro and in vivo[J]. Advances in Pharmacological and Pharmaceutical Sciences, 2011, 2011(1): 691928.
[7] Caslin H L, Bolus W R, Thomas C, et al. Bovine Serum Albumin Elicits IL-33–Dependent Adipose Tissue Eosinophilia: Potential Relevance to Ovalbumin-induced Models of Allergic Disease[J]. ImmunoHorizons, 2023, 7(12): 842-852.
Bovine Serum Albumin(Fatty Acid & lgG Free)是一种由583个氨基酸残基组成的球状蛋白质和寡核苷酸结合蛋白，由三个同源的全α结构域组成 [1]。Bovine Serum Albumin可阻断寡核苷酸与细胞的整体结合 [2]。Bovine Serum Albumin常用作免疫组织化学中的阻滞剂，也适用于蛋白质、酶系统、蛋白质标准、杂交以及蛋白酶敏感性免疫测定 [3-4]。
在体外，在葡萄糖病理生理浓度下，Bovine Serum Albumin（0.373mM; 8、16和24h）处理显著增加了人类成熟脂肪细胞的LDH活性，以及增加了细胞中的羰基含量和异前列腺素的释放 [5]。Bovine Serum Albumin（1mg/mL; 18h）预处理剂量依赖性增加了脂多糖（LPS）刺激的小鼠腹膜巨噬细胞（RAW264.7z）中NF-κB启动子活性和TNF-α的基因表达[6]。
在体内，Bovine Serum Albumin（10mg/kg/day; i.p.）单剂量治疗显著减弱了内毒素休克模型小鼠的促炎反应，降低了小鼠血浆中的TNF-α含量 [6]。Bovine Serum Albumin（0.1%, 250μl; twice weekly for 4 or 8 weeks; i.p.）处理显著增多了饮食诱导肥胖的小鼠模型脂肪组织中的嗜酸性粒细胞数量并促进了2型细胞因子的基因表达以及IgE的生成 [7]。
| Cell experiment [1]: | |
Cell lines | Human mature adipocytes |
Preparation Method | Digested adipose tissues were filtered through a 80μM membrane and submitted to a 30s centrifugation at 1000rpm, in order to separate mature adipocytes, which float in the supernatant. About 300 000 cells were seeded in a well of a 24-well plate containing 500μl of RPMI medium, supplemented with 1.25% l-glutamine and 2% penicillin/streptomycin. Mature adipose cells were grown in a CO2 incubator (5% CO2/95% air) at 37°C. Cells were incubated with PBS or 0.37mM Bovine Serum Albumin during 16h at 37°C. Cell viability was estimated by measuring the lactate dehydrogenase (LDH) activity in the cell medium using an in vitro toxicology assay kit and accordingly to the manufacturer’s instructions. |
Reaction Conditions | 0.37mM; 16h |
Applications | Treatment with Bovine Serum Albumin significantly increased the LDH activity of human mature adipocytes. |
| Animal experiment [2]: | |
Animal models | C57Bl/6 mice (Murine Model of Endotoxin Shock) |
Preparation Method | C57Bl/6 mice, 20–25g body weight, were acclimatized for 7 days prior to surgical manipulation and maintained on 12-h light/dark cycles with access to food and water ad libitum. Briefly, mice were preconditioned with Bovine Serum Albumin, 10mg/kg body wt or vehicle, administered via intraperitoneal (i.p.) injection and were returned to their cages. After 18h, 60mg/kg body weight of lipopolysaccharide (LPS) was administered via i.p. injection. Mice were anesthetized 6 hours later with isoflurane, and blood was obtained via direct cardiac puncture and centrifuged at 5,000g × 10 minutes in EDTA-calcium vacutainer. The plasma was then collected and stored at −80°C until further analysis. Plasma concentration of tumor necrosis factor- (TNF-) α was measured via commercially available enzyme-linked immunosorbent assay kits using the protocol recommended by the manufacturer. |
Dosage form | 10mg/kg/day; single dose; i.p. |
Applications | Bovine Serum Albumin treatment significantly attenuated the inflammatory response and reduced the content of TNF-α in the plasma of mice. |
References: | |
| Cas No. | 9048-46-8 | SDF | |
| 别名 | BSA，牛血清白蛋白来源于牛血清,牛血清白蛋白,白蛋白牛血清 | ||
| 分子式 | 分子量 | 66kDa | |
| 溶解度 | H2O : 250 mg/mL (Need ultrasonic) | 储存条件 | Store at 4 ℃ |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 15.1515 mL | 75.7576 mL | 151.5152 mL |
| 5 mM | 3.0303 mL | 15.1515 mL | 30.303 mL |
| 10 mM | 1.5152 mL | 7.5758 mL | 15.1515 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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Purity: >98.00%
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