Polymyxin B
(Synonyms: 多粘菌素B) 目录号 : GC19581
多粘菌素B（PMB）是一种强效抗生素，可结合并中和LPS（脂多糖）。
Cas No.:1404-26-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Biological Activity: 8282IU/mg
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment [1]: |
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Cell lines |
HK-2 cells |
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Preparation method |
HK-2 cells were incubated with polymyxin B (12.5, 50 and 100 μM) for 6 or 24 h and stained with DAPI (10 μg/mL) for assessment of micronuclei. |
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Reaction Conditions |
12.5, 50 and 100 μM, 6 or 24 h |
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Applications |
Human proximal tubular cells were treated with polymyxin B (12.5-100 μM) for up to 24 h and showed a significant increase in micronuclei frequency, as well as abnormal mitotic events. |
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Animal experiment [2]: |
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Animal models |
Guinea pigs |
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Preparation method |
Guinea pigs were transnasally treated with 75 µg/kg of polymyxin-B or vehicle twice a week for a total of 3 weeks. Guinea pigs were surgically cannulated and artificially ventilated 24 h after the last administration of polymyxin-B or vehicle. Ten minutes after the installation of artificial ventilation, ascending doses of methacholine, acetylcholine or histamine were inhaled for 20 s at intervals of 5 min. Subsequent study was conducted 20 min after treatment of 60 mg/kg of indomethacin in the same manner. Final study was conducted in naive guinea pigs after single inhalation of 75 µg/mL of polymyxin B. |
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Dosage form |
75 µg/kg, transnasally |
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Applications |
The proportion of eosinophils in bronchoalveolar lavage fluid significantly increased in guinea pigs treated with polymyxin-B compared with vehicle. Bronchial responsiveness to inhaled methacholine, acetylcholine and histamine was significantly decreased by the polymyxin-B treatment. This protective effect induced by polymyxin B was abolished by pretreatment of indomethacin. A significant increase in bronchial responsiveness was observed after a single inhalation of polymyxin B. |
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References: [1] Yun B, et al. Polymyxin B causes DNA damage in HK-2 cells and mice. Arch Toxicol. 2018 Jul;92(7):2259-2271. [2] Ishiura Y, et al. In vivo airway eosinophil accumulation induced by polymyxin-B reduces bronchial responsiveness in guinea pigs. Clin Exp Allergy. 2001 Apr;31(4):644-51. |
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Polymyxin B (PMB) is a potent antibiotic that binds to and neutralizes LPS (lipopolysaccharides)[1].
Polymyxin B againsts all K. pneumoniae strains for positive K. pneumoniae carbapenemase (KPC) genes with MIC of 16 to 128 μg/ml[2]. In vitro,10 µ/mL Polymyxin B was able to neutralize the effect of endotoxin, as contaminant in S. mansoni recombinant antigens produced in E. coli, in inducing TNF-alpha and IL-10 production[1]. The MIC50 and MIC90 values of polymyxin B against 217 strains of carbapenem-resistant A. baumannii were 0.5 and 1 mg/l, respectively[3]. The MIC values for polymyxin B against MRSA (Meticillin-resistant Staphylococcus aureus) was in the range of 8-64 μg/mL, while the MIC values for polymyxin B against MRSP (meticillin-resistant Staphylococcus pseudintermedius) was in the range of 0.25-4 μg/mL[4].
In vivo, mice were treated with 72 mg/kg obviously increased the level of γH2AX foci (indicative of double stranded breaks)[5]. In vivo, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibite antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood[6].
References:
[1] Cardoso LS, et al. Polymyxin B as inhibitor of LPS contamination of Schistosoma mansoni recombinant proteins in human cytokine analysis. Microb Cell Fact. 2007 Jan 3;6:1.
[2] Elemam A, et al. In vitro evaluation of antibiotic synergy for polymyxin B-resistant carbapenemase-producing Klebsiella pneumoniae. J Clin Microbiol. 2010 Oct;48(10):3558-62.
[3] Thamlikitkul V, et al. In vitro activity of polymyxin B against carbapenem-resistant Acinetobacter baumannii. J Med Assoc Thai. 2014 Dec;97(12):1254-8.
[4] Boyen F, et al. In vitro antimicrobial activity of miconazole and polymyxin B against canine meticillin-resistant Staphylococcus aureus and meticillin-resistant Staphylococcus pseudintermedius isolates. Vet Dermatol. 2012 Aug;23(4):381-5, e70.
[5] Yun B, et al. Polymyxin B causes DNA damage in HK-2 cells and mice. Arch Toxicol. 2018 Jul;92(7):2259-2271.
[6] de Souza GH, et al. Synergistic interaction of polymyxin B with carvacrol: antimicrobial strategy against polymyxin-resistant Klebsiella pneumoniae. Future Microbiol. 2024 Feb 8.
多粘菌素B（PMB）是一种强效抗生素，可结合并中和LPS（脂多糖）[1]。
多粘菌素B对所有K. pneumoniae carbapenemase（KPC）基因阳性的菌株均具有抗药性，MIC为16至128μg/ml[2]。在体外，10 µ/mL多粘菌素B能够中和内毒素诱导TNF-α和IL-10产生的作用[1]，内毒素是在大肠杆菌中产生的S. mansoni重组抗原中的污染物。多粘菌素B对217株carbapenem-resistant A. baumannii 的MIC50和MIC90值分别为0.5和1 mg/l[3]。多粘菌素B对MRSA（耐甲氧西林Staphylococcus aureus）的MIC值在8-64 μg/mL范围内，而多粘菌蛋白B对MRSP（耐甲羟西林Staphylococcus pseudintermedius）在0.25-4 μg/mL范围内[4]。
在体内，用72 mg/kg处理的小鼠明显增加了γH2AX病灶的水平（表明双链断裂）[5]。在体内，香芹酚10 mg/kg加多粘菌素B 2 mg/kg在小鼠感染模型中表现出抗菌活性，从而提高存活率并显著降低血液中的细菌载量[6]。
| Cas No. | 1404-26-8 | SDF | |
| 别名 | 多粘菌素B | ||
| 分子式 | 分子量 | ||
| 溶解度 | H2O : 33.33 mg/mL (ultrasonic and adjust pH to 2 with HCl) | 储存条件 | Store at -20℃ |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。