Asiaticoside
(Synonyms: 积雪草苷) 目录号 : GN10534
Asiaticoside是从Centella asiatica中提取的一种五环三萜苷。
Cas No.:16830-15-2
Sample solution is provided at 25 µL, 10mM.
Asiaticoside is a pentacyclic triterpenoid glycoside extracted from Centella asiatica [1]. Asiaticoside activates the Smad signaling pathway (specifically in a TGF-β receptor I kinase-independent manner), promoting the synthesis of type I collagen by fibroblasts, thereby strengthening skin structure and wound healing [2-3]. Asiaticoside is commonly used in wound healing and skin disease treatment [4].
In HCT116 cells, Asiaticoside (0.1-2µM; 24-72h) significantly reduced cell viability in a time- and dose-dependent manner [5]. In human brain microvascular endothelial cells, Asiaticoside (50µM; 24h) effectively attenuated Aβ1-42-induced cell growth inhibition [6].
In Swiss mice, Asiaticoside (5-40mg/kg; po; single administration) treatment increased the percentage of mice entering the open arm and the time they stayed in the open arm [7], In Swiss mice Asiaticoside (10-20mg/kg; po; single administration) significantly shortened the immobility time of mice in the tail suspension test [8].
References:
[1]. Shukla A, Rasik A M, Jain G K, et al. In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica[J]. Journal of ethnopharmacology, 1999, 65(1): 1-11.
[2]. Tang B, Zhu B, Liang Y, et al. Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts[J]. Archives of dermatological research, 2011, 303(8): 563-572.
[3]. Lee J, Jung E, Kim Y, et al. Asiaticoside induces human collagen I synthesis through TGFβ receptor I kinase (TβRI kinase)-independent Smad signaling[J]. Planta medica, 2006, 72(04): 324-328.
[4]. Bandopadhyay S, Mandal S, Ghorai M, et al. Therapeutic properties and pharmacological activities of asiaticoside and madecassoside: A review[J]. Journal of cellular and molecular medicine, 2023, 27(5): 593-608.
[5]. Zhou X, Ke C, Lv Y, et al. Asiaticoside suppresses cell proliferation by inhibiting the NF‑κB signaling pathway in colorectal cancer[J]. International Journal of Molecular Medicine, 2020, 46(4): 1525-1537.
[6]. Song D, Jiang X, Liu Y, et al. Asiaticoside attenuates cell growth inhibition and apoptosis induced by Aβ1-42 via inhibiting the TLR4/NF-κB signaling pathway in human brain microvascular endothelial cells[J]. Frontiers in pharmacology, 2018, 9: 28.
[7]. Chen S W, Wang W J, Li W J, et al. Anxiolytic-like effect of asiaticoside in mice[J]. Pharmacology Biochemistry and Behavior, 2006, 85(2): 339-344.
[8]. Liang X, Huang Y N, Chen S W, et al. Antidepressant-like effect of asiaticoside in mice[J]. Pharmacology Biochemistry and Behavior, 2008, 89(3): 444-449.
Asiaticoside是从Centella asiatica中提取的一种五环三萜苷 [1]。Asiaticoside能够激活Smad信号通路（特别是以TGF-β受体I激酶非依赖的方式），促进成纤维细胞合成I型胶原蛋白，从而增强皮肤结构和促进伤口愈合 [2-3]。Asiaticoside常用于伤口愈合和皮肤病治疗 [4]。
在HCT116细胞中，Asiaticoside（0.1-2µM；24-72h）显著降低细胞活力，且呈时间和剂量依赖性 [5]。在人脑微血管内皮细胞中，Asiaticoside（50µM；24h）有效减弱了Aβ1-42诱导的细胞生长抑制 [6]。
在瑞士小鼠中，Asiaticoside（5-40mg/kg；po；单次给药）治疗增加了小鼠进入开放臂的百分比以及它们在开放臂中停留的时间 [7]，在瑞士小鼠中，Asiaticoside（10-20mg/kg；po；单次给药）显著缩短了小鼠在悬尾试验中的不动时间 [8]。
| Cell experiment [1]: | |
Cell lines | HCT116 cells |
Preparation Method | Cell viability was assessed using a CCK-8 assay. HCT116 cells in the logarithmic growth phase were digested and seeded into 96-well plates at a density of 5×103 cells/well followed by treatment with various concentrations of Asiaticoside (0.1, 0.5 and 2µM) at 37℃. Cells treated with 0.3% DMSO were used as the negative controls. Subsequently, 10% CCK-8 reagent was added to each well at different time intervals (24, 48 and 72h), and cells incubated for 2h at 37℃. The absorbance was then measured using a microplate reader at a wavelength of 450nm. |
Reaction Conditions | 0.1-2µM; 24-72h |
Applications | Asiaticoside significantly reduced cell viability in a time- and dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Swiss mice |
Preparation Method | The elevated plus-maze consists of four arms (30 × 5cm) elevated 45cm above the floor, with each arm positioned at 90° relative to the adjacent arms. The two enclosed arms had 30cm walls and to facilitate grip on the open arms these included a raised edge of 0.25cm. Open and closed arms were connected via a central area (5 × 5cm) to form a plus sign. The maze floor was constructed of black Plexiglas and the wall of the enclosed arms was constructed of clear Plexiglas. Four 25-W red fluorescent lights arranged as a cross at 100cm above the maze were used as the source of illumination. Mice (n= 9-10 per group) were randomly assigned (with a slight adjustment for matched body-weight) to eight experimental groups (vehicle-control, 0.5, 1, 2mg/kg diazepam or 5, 10, 20, 40mg/kg Asiaticoside). Drug was administration PO 60min prior to the test. Testing commenced by placing a mouse on the central platform of the maze facing an open arm. The number of entries into and the time spent on each of the two types of arms were recorded during the 5-min trial. An arm entry was defined as all four paws having crossed the dividing line between an arm and the central area. The plus-maze was thoroughly cleaned after each trial. |
Dosage form | 5-40mg/kg; po; single administration |
Applications | Asiaticoside treatment increased the percentage of mice entering the open arm and the time they stayed in the open arm. |
References: | |
| Cas No. | 16830-15-2 | SDF | |
| 别名 | 积雪草苷 | ||
| 化学名 | Asiaticoside | ||
| Canonical SMILES | CC1CCC2(CCC3(C(=CCC4C3(CCC5C4(CC(C(C5(C)CO)O)O)C)C)C2C1C)C)C(=O)OC6C(C(C(C(O6)COC7C(C(C(C(O7)CO)OC8C(C(C(C(O8)C)O)O)O)O)O)O)O)O | ||
| 分子式 | C48H78O19 | 分子量 | 959.12 |
| 溶解度 | ≥ 44.6mg/mL in DMSO | 储存条件 | Store at 2-8°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0426 mL | 5.2131 mL | 10.4262 mL |
| 5 mM | 208.5 μL | 1.0426 mL | 2.0852 mL |
| 10 mM | 104.3 μL | 521.3 μL | 1.0426 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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