Ardisiacrispin B
(Synonyms: 百两金皂苷B) 目录号 : GC65163
Ardisiacrispin B是从传统中药植物紫金牛属植物中分离得到的天然三萜皂苷化合物 。
Cas No.:112766-96-8
Sample solution is provided at 25 µL, 10mM.
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Ardisiacrispin B is a natural triterpenoid saponin compound isolated from the traditional Chinese medicinal plant Ardisia [1]. Ardisiacrispin B exhibits cytotoxic effects in multifactorial drug-resistant cancer cells via ferroptosis and apoptotic cell death [2]. Ardisiacrispin B has anticancer, anti-inflammatory and antioxidant effects [3-4].
In CCRF-CEM cells, Ardisiacrispin B (0.005-6.4μM; 72h) induced apoptosis in CCRF-CEM cells via activation of inititator caspases 8 and 9 and effector caspase 3/7, alteration of MMP and increase in ROS production [2]. In RAW264.7 cells, Ardisiacrispin B (0.125μM, 0.5μM, 2μM; 24h) has a good anti-inflammatory effect on LPS-induced RAW264.7 cells, strongly inhibits the release of NO, TNF-α and IL-1β in cells, and significantly inhibits the expression of PI3K, P-PI3K, AKT and P-AKT [5]. In A549 cells, Ardisiacrispin B (2.5μM, 5μM, 10μM, 20μM,50μM; 48h) displayed moderate cytotoxicity against A549 with an IC50 value of 8.7μM [6].
In sprague dawley rats, Ardisiacrispin B (50mg/kg; ig; twice a day for 3 consecutive days) is converted into water-soluble components in the body mainly through metabolic processes such as oxidation, dehydration, and glycoside conjugation [5].
References:
[1]. Jansakul C, Baumann H, Kenne L, et al. Ardisiacrispin A and B, two utero-contracting saponins from Ardisia crispa. Planta medica. 1987 Oct; 53(05): 405-409.
[2]. Mbaveng AT, Ndontsa BL, Kuete V, et al. A naturally occuring triterpene saponin ardisiacrispin B displayed cytotoxic effects in multi-factorial drug resistant cancer cells via ferroptotic and apoptotic cell death. Phytomedicine. 2018 Apr 1; 43: 78-85.
[3]. Tao HH, Zhou YQ, Wei X, et al. Anti‐inflammatory activity of a new lactone isolated from the leaves of Ardisia crenata Sims. Chemistry & Biodiversity. 2024 Jan; 21(1): e202300983.
[4]. Xie Q, Shama R, Yu J, et al. Ethnobotanical study of Zhuang medicinal herbs of Ardisia: variety systematization, traditional uses, phytochemistry, pharmacology, clinical application, and toxicity. Journal of Pharmacy and Pharmacology. 2024 Apr 1; 76(4): 327-353.
[5]. Zhou W, Yang G, Wen Y, et al. Metabolites-based network pharmacology to preliminarily verify in vitro anti-inflammatory effect of Ardisiacrispin B. International Journal of Molecular Sciences. 2023 Dec 2; 24(23): 17059.
[6]. Dai LM, Huang RZ, Zhang B, et al. Cytotoxic triterpenoid saponins from Lysimachia foenum-graecum. Phytochemistry. 2017 Apr 1; 136: 165-174.
Ardisiacrispin B是从传统中药植物紫金牛属植物中分离得到的天然三萜皂苷化合物 [1]。Ardisiacrispin B通过诱导铁死亡和凋亡性细胞死亡，在多因素耐药癌细胞中表现出细胞毒作用 [2]。Ardisiacrispin B具有抗癌、抗炎和抗氧化作用 [3-4]。
在CCRF-CEM细胞中，Ardisiacrispin B（0.005-6.4μM；72h）通过激活启动子caspase 8和9 以及效应子caspase 3/7、改变MMP和增加ROS生成，诱导CCRF-CEM细胞凋亡 [2]。在RAW264.7细胞中，Ardisiacrispin B（0.125μM、0.5μM、2μM；24h）对LPS诱导的RAW264.7细胞具有良好的抗炎作用，强效抑制细胞内NO、TNF-α和IL-1β的释放，并显著抑制PI3K、P-PI3K、AKT和P-AKT的表达 [5]。在A549细胞中，Ardisiacrispin B（2.5μM、5μM、10μM、20μM、50μM；48h）对A549细胞表现出中等细胞毒性，IC50值为8.7μM [6]。
在sprague dawley大鼠中，Ardisiacrispin B（50mg/kg；ig；每天2次，连续3天）在体内主要通过氧化、脱水、糖苷结合等代谢过程转化为水溶性成分 [5]。
| Cell experiment [1]: | |
Cell lines | CCRF-CEM cells |
Preparation Method | Leukemia CCRF-CEM cells were maintained in RPMI 1640 medium supplemented with 10% fetal calf serum in a humidified 5% CO2 atmosphere at 37℃. The cytotoxicity of Ardisiacrispin B and the reference drug doxorubicin (positive control) were evaluated. Dimethyl sulfoxide (DMSO) was used to solubilize the samples and tested as a negative control. The highest concentration of DMSO was less than 0.1%. The concentration range of ardisiacrispin B tested in CCRF-CEM was 0.005-6.4μM. |
Reaction Conditions | 0.005-6.4μM; 72h |
Applications | Ardisiacrispin B induced apoptosis in CCRF-CEM cells via activation of inititator caspases 8 and 9 and effector caspase 3/7, alteration of MMP and increase in ROS production. |
| Animal experiment [2]: | |
Animal models | Sprague dawley rats |
Preparation Method | Twenty SPF-grade male sprague dawley rats weighing 200 ± 20g (8-10 weeks old). Once the rats entered the animal house, they were managed in packs of five per cage, fed with standard chow, and provided with fresh, purified water that was replaced daily. The animal house was well lit with a 12-hour day/night cycle, well ventilated, and maintained at a room temperature of 22 ± 2℃ and a relative humidity of 15–60%, and it required regular disinfection. Prior to the experiment, rats were adaptively reared in the laboratory for 7 days. These animals were randomly divided into a blank control group and an Ardisiacrispin B administrated group. The blank control group was gavaged water, and the Ardisiacrispin B administered group was gavaged with 50mg/kg of Ardisiacrispin B aqueous solution (concentration of 6mg/mL), twice a day for 3 consecutive days. |
Dosage form | 50mg/kg; ig; twice a day for 3 consecutive days |
Applications | Ardisiacrispin B is converted into water-soluble components in the body mainly through metabolic processes such as oxidation, dehydration, and glycoside conjugation. |
References: | |
| Cas No. | 112766-96-8 | SDF | Download SDF |
| 别名 | 百两金皂苷B | ||
| 分子式 | C53H86O22 | 分子量 | 1075.24 |
| 溶解度 | DMSO: 100 mg/mL | 储存条件 | Store at -20°C, away from moisture and light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.93 mL | 4.6501 mL | 9.3002 mL |
| 5 mM | 0.186 mL | 0.93 mL | 1.86 mL |
| 10 mM | 0.093 mL | 0.465 mL | 0.93 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet