Ampicillin sodium
(Synonyms: 氨苄西林钠; D-(-)-α-Aminobenzylpenicillin sodium salt) 目录号 : GC10036
氨苄青霉素钠是一种广谱β-内酰胺类抗生素，对多种革兰氏阳性菌和革兰氏阴性菌有效
Cas No.:69-52-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Animal experiment: |
Mice: Ampicillin is dissolved in normal saline. Male C57BL/6 mice were anesthetized with halothane and subjected to bilateral common carotid artery occlusion for 40 min. Before transient forebrain ischemia, ampicillin (200 mg/kg, intraperitoneally [i.p.]) or penicillin G (6,000 U/kg or 20,000 U/kg, i.p.) was administered daily for 5 days. In the control animals, saline was administered at the same volume and time schedule[3]. |
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References: [1]. Chopra SL, et al. Effect of Ampicillin on E. Coli of Swine Origin. Can J Comp Med Vet Sci. 1963 Sep;27(9):223-7. |
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Ampicillin sodium is a broad-spectrum beta-lactam antibiotic against a variety of gram-positive and gram-negative bacteria.
Ampicillin inhibits the growth of E. coli of swine origin in a dose-dependent manner. The effective inhibitory concentration of Ampicillin was 2.5 uG/mL[1].
Ampicillin is very effective in alleviating the symptoms of hemorrhagic enteritis in a 11-week old pig[1]. Ampicillin produces maximum concentrations in bile twice as high as in serum. The peak concentration of ampicillin after an oral dose is as twice as high in portal blood as in peripheral blood[2]. Ampicillin provides neuroprotection against ischemia-reperfusion brain injury. Ampicillin reduces the activities of MMPs and increases the expression level of GLT-1. Pretreatment with ampicillin significantly reduces medial hippocampal cell death following global forebrain ischemia[3].
氨苄青霉素钠是一种广谱β-内酰胺类抗生素，对多种革兰氏阳性菌和革兰氏阴性菌有效。
氨苄青霉素剂量依赖性地抑制了猪源大肠杆菌的生长。氨苄青霉素的有效抑菌浓度为2.5微克/毫升[1]。
氨苄青霉素对11周龄猪的出血性肠炎症状具有很好的缓解作用[1]。氨苄青霉素在胆汁中的峰值浓度是血清的两倍。口服后，氨苄青霉素在门静脉血中的峰值浓度是外周血的两倍[2]。氨苄青霉素对缺血再灌注性脑损伤具有神经保护作用。氨苄青霉素降低了MMPs的活性并增加了GLT-1的表达水平。氨苄青霉素预处理显著减少了全脑前动脉缺血后中央海马细胞死亡[3]。
References:
[1]. Chopra SL, et al. Effect of Ampicillin on E. Coli of Swine Origin. Can J Comp Med Vet Sci. 1963 Sep;27(9):223-7.
[2]. Lund B, et al. Ampicillin in portal and peripheral blood and bile after oral administration of ampicillin andpivampicillin. Eur J Clin Pharmacol. 1974;7(2):133-5.
[3]. Lee KE, et al. The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia. Korean J Physiol Pharmacol. 2016 Mar;20(2):185-92.
| Cas No. | 69-52-3 | SDF | |
| 别名 | 氨苄西林钠; D-(-)-α-Aminobenzylpenicillin sodium salt | ||
| 化学名 | sodium;(2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate | ||
| Canonical SMILES | CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)[O-])C.[Na+] | ||
| 分子式 | C16H18N3NaO4S | 分子量 | 371.4 |
| 溶解度 | ≥ 73.6 mg/mL in DMSO, ≥ 75.2 mg/mL in EtOH, ≥ 18.57 mg/mL in Water | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6925 mL | 13.4626 mL | 26.9251 mL |
| 5 mM | 0.5385 mL | 2.6925 mL | 5.385 mL |
| 10 mM | 0.2693 mL | 1.3463 mL | 2.6925 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Ampicillin sodium and nephrotoxicity
Arch Intern Med 1977 Jun;137(6):812.869655
Unasyn (Ampicillin sodium/sulbactam sodium)
Gastroenterol Nurs 1993 Aug;16(1):36-8.8399439 10.1097/00001610-199308000-00011
Unasyn is a combination drug used for the treatment of infections as well as a prophylactic intervention prior to surgical or endoscopic interventions. Unasyn is a broad spectrum antibiotic of the penicillin family that inhibits beta-lactamases, ensuring activity of the ampicillin against resistant organisms. Peak serum levels of Unasyn are reached in 15 min to 1 hr after an IV infusion of 15 min.
Stability of aztreonam and ampicillin sodium-sulbactam sodium in 0.9% sodium chloride injection
Am J Hosp Pharm 1994 Apr 1;51(7):901-4.8017440
The stability of aztreonam, Ampicillin sodium, and sulbactam sodium admixed in 0.9% sodium chloride injection and stored at room temperature and under refrigeration was studied. Each of the following admixtures was prepared in 0.9% sodium chloride injection: (1) aztreonam 10 mg/mL; (2) ampicillin 20 mg/mL (as the sodium salt) and sulbactam 10 mg/mL (as the sodium salt); and (3) aztreonam 10 mg/mL, ampicillin 20 mg/mL, and sulbactam 10 mg/mL. Three minibags of each admixture were stored at room temperature and three were refrigerated. Every 12 hours, up to 96 hours, the admixtures were visually inspected and 5-mL samples were withdrawn for high-performance liquid chromatography and pH testing. No color change or precipitation was observed in any sample. In admixtures containing ampicillin, ampicillin was the first or only drug to lose more than 10% of initial concentration. In the ampicillin-sulbactam admixture, ampicillin was stable for 32 hours at room temperature and 68 hours refrigerated. In the aztreonam-ampicillin-sulbactam admixture, ampicillin was stable for 30 hours at room temperature and 94 hours refrigerated. Aztreonam 10 mg/mL, ampicillin 20 mg/mL (as the sodium salt), and sulbactam 10 mg/mL (as the sodium salt) in 0.9% sodium chloride injection were stable in combination for up to 30 hours at room temperature and 94 hours under refrigeration.
Ampicillin sodium: Isolation, identification and synthesis of the last unknown impurity after 60 years of clinical use
J Pharm Biomed Anal 2020 Nov 30;191:113584.32889349 10.1016/j.jpba.2020.113584
Ampicillin, discovered in 1958, was the first broad spectrum semisynthetic penicillin introduced into the market. Despite its wide use not all the impurities have been identified to date. Herein, the last unknown impurity present in commercially available medicines was isolated and identified. This impurity that accounts up to 0.8 in area % by HPLC (EP 10.0) in the Reference Listed Drugs (RLD) was characterized and identified to be the 16-keto penicillin G. The structure was confirmed by comparison with a chemically synthesized sample. The determination of the Relative Response Factor (RRF) of the impurity respect to the parent drug allowed to recalculate the real amount that is consistently below the reporting threshold.
Determination of Ampicillin sodium using the cupric oxide nanoparticles-luminol-H2 O2 chemiluminescence reaction
Luminescence 2014 Sep;29(6):679-83.24254330 10.1002/bio.2606
A simple and sensitive chemiluminescence (CL) method has been developed for the determination of Ampicillin sodium at submicromolar levels. The method is based on the inhibitory effect of Ampicillin sodium on the cupric oxide nanoparticles (CuO NPs)-luminol-H2 O2 CL reaction. Experimental parameters affecting CL inhibition including concentrations of CuO NPs, luminol, H2 O2 and NaOH were optimized. Under optimum conditions, the calibration plot was linear in the analyte concentration range 4.0 脳 10(-7) -4.0 脳 10(-6) mol/L. The limit of detection was 2.6 脳 10(-7) mol/L and the relative standard deviation (RSD) for six replicate determinations of 1 脳 10(-6) mol/L Ampicillin sodium was 4.71%. Also, X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis were employed to characterize the CuO NPs. The utility of the proposed method was demonstrated by determining Ampicillin sodium in pharmaceutical preparation.