Alteminostat
(Synonyms: CKD-581) 目录号 : GC67984Alteminostat (CKD-581) 是一种有效的 HDAC 抑制剂。Alteminostat 通过组蛋白 H3 和微管蛋白乙酰化抑制 I-II 类 HDAC 家族。Alteminostat 可用于淋巴瘤和多发性骨髓瘤的研究。
Cas No.:1246374-97-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Alteminostat (CKD-581) is a potent HDAC inhibitor. Alteminostat inhibits the class I-II HDAC family via histone H3 and tubulin acetylation. Alteminostat can be used for lymphoma and multiple myeloma research[1].
Alteminostat (CKD-581; 1 nM-10 μM; 72 hours) treatment potently reduces cell viability in all four lymphoma cell lines in a concentration-dependent manner. The IC50 values of Alteminostat in SU-DHL-4, OCI-LY1, SU-DHL-2, and U2932 cells are 1.31 nM, 36.91 nM, 1.18 nM, and 31.99 nM, respectively[1].
Alteminostat (CKD-581; 10-300 nM; 24 hours) treatment decreases the expression of BCL-6 as well as BCL-2 in cells[1].
Alteminostat (CKD-581; 30-300 nM; 24 h) treatment results in γH2AX accumulation and PARP1 cleavage in SU-DHL-4, OCI-LY1, SU-DHL-2, and U2932 cells. Alteminostat decreases the protein levels of BCL-XL and MCL-1 in a concentration-dependent manner in OCI-LY1 cells[1].
Alteminostat (CKD-581; 10-300 nM; 6 hours) treatment increases the acetylation of histone H3 in SU-DHL-2 cells. And tubulin acetylation also increased with 10 nM CKD-581. CKD-581 increased the acetylation of target molecules by inhibiting class I-II HDACs in lymphoma cells[1].
Cell Viability Assay[1]
| Cell Line: | SU-DHL-4, OCI-LY1, SU-DHL-2, and U2932 cells |
| Concentration: | 1 nM-10 μM |
| Incubation Time: | 72 hours |
| Result: | Potently reduced cell viability in all four lymphoma cell lines in a concentration-dependent manner. |
Western Blot Analysis[1]
| Cell Line: | SU-DHL-4 and OCI-LY1 cells |
| Concentration: | 10 nM, 30 nM, 100 nM, 300 nM |
| Incubation Time: | 24 hours |
| Result: | Decreased the expression of BCL-6 as well as BCL-2 in cells. |
Alteminostat (CKD-581; 20-40 mg/kg; ntraperitoneal injection; twice a week; for 4 weeks) treatment partially but significantly suppresses tumor growth in SU-DHL-4 xenograft mice[1].
| Animal Model: | Male NOD.CB17 SCID injected with SU-DHL-4 cells[1] |
| Dosage: | 20 mg/kg or 40 mg/kg |
| Administration: | Intraperitoneal injection; twice a week; for 4 weeks |
| Result: | Partially but significantly suppressed tumor growth. |
[1]. Soo Jin Kim, et al. Anti-Cancer Effects of CKD-581, a Potent Histone Deacetylase Inhibitor against Diffuse Large B-Cell Lymphoma. Int J Mol Sci. 2020 Jun 19;21(12):4377.
| Cas No. | 1246374-97-9 | SDF | Download SDF |
| 别名 | CKD-581 | ||
| 分子式 | C27H36N6O3 | 分子量 | 492.61 |
| 溶解度 | 储存条件 | Store at -20°C, protect from light | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.03 mL | 10.15 mL | 20.3 mL |
| 5 mM | 0.406 mL | 2.03 mL | 4.06 mL |
| 10 mM | 0.203 mL | 1.015 mL | 2.03 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。