Acetosyringone
(Synonyms: 乙酰丁香酮) 目录号 : GC33508
Acetosyringone是植物受伤组织分泌的一种酚类物质，是VirA/VirG双组分系统的特异性激动剂。
Cas No.:2478-38-8
Sample solution is provided at 25 µL, 10mM.
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Acetosyringone is a phenolic compound secreted by plant wounded tissues and is a specific agonist of the VirA/VirG two-component system[1]. Acetosyringone induces the activation of the VirA gene in Agrobacterium tumefaciens, triggering the autophosphorylation of VirA and the subsequent transfer of the phosphate group to the response regulator VirG. The phosphorylated VirG then activates the expression of all virulence (vir) genes on the Ti plasmid, thereby driving the processing, transfer, and stable integration of T-DNA into the plant genome[2]. Acetosyringone is widely used in Agrobacterium-mediated genetic transformation and plant antibacterial mechanism studies[3][4].
Pre-induction of Agrobacterium strains harboring pMDC45 with Acetosyringone (100μM; 24h) and 10mM D-glucose in TAP medium significantly enhanced the integration efficiency of GFP and HPT transgenes in Dunaliella salina during in vivo co-cultivation[5]. Incubation of G. intraradices spores with Acetosyringone (200μM, 2h) resulted in an overall increase in hyphal respiration and significantly induced the rapid expression of symbiotic related genes such as GiBP1[6].
Co-incubating Agrobacterium with 500μM acetosyringone for 2–4h, infiltrating the mixture into Nicotiana benthamiana leaves and sampling at 4 dpi increased transient GUS expression about five-fold[7].
References:
[1] Xi J, Patel M, Dong S, Que Q, Qu R. Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus. BMC Biotechnol. 2018;18(1):48.
[2] Shaw CH. Swimming against the tide: chemotaxis in Agrobacterium. Bioessays. 1991;13(1):25-29.
[3] Sheikholeslam SN, Weeks DP. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Mol Biol. 1987;8(4):291-298.
[4] Szatmári Á, Móricz ÁM, Schwarczinger I, et al. A pattern-triggered immunity-related phenolic, acetosyringone, boosts rapid inhibition of a diverse set of plant pathogenic bacteria. BMC Plant Biol. 2021;21(1):153.
[5] Srinivasan R, Gothandam KM. Synergistic Action of D-Glucose and Acetosyringone on Agrobacterium Strains for Efficient Dunaliella Transformation. PLoS One. 2016;11(6):e0158322.
[6] Flores-Gómez E, Gómez-Silva L, Ruiz-Medrano R, Xoconostle-Cázeres B. Role of acetosyringone in the accumulation of a set of RNAs in the arbuscular mycorrhiza fungus Glomus intraradices. Int Microbiol. 2008;11(4):275-282.
[7] Norkunas K, Harding R, Dale J, Dugdale B. Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana. Plant Methods. 2018;14:71.
Acetosyringone是植物受伤组织分泌的一种酚类物质，是VirA/VirG双组分系统的特异性激动剂[1]。Acetosyringone可诱导根癌农杆菌 （Agrobacterium tumefaciens）的VirA基因 活化，触发VirA自磷酸化并将磷酸基团传递给应答调节蛋白VirG；磷酸化的VirG随即激活Ti质粒上所有毒力（vir）基因的表达，进而驱动T-DNA的加工、转运及在植物基因组中的稳定整合[2]。Acetosyringone广泛应用于农杆菌介导的遗传转化和植物抗菌机制研究[3][4]。
用100μM Acetosyringone和10mM D-葡萄糖在TAP培养基中预诱导携带pMDC45的农杆菌菌株24小时，显著提高了GFP和HPT转基因在Dunaliella salina体内共培养中的整合效率[5]。 Glomus intraradices孢子与200μM Acetosyringone 共培养2小时导致菌丝呼吸速率整体增加，并显著诱导了与共生相关的基因（如GiBP1）的快速表达[6]。
将农杆菌与500μM Acetosyringone 共培养2–4小时，随后将混合物浸润到Nicotiana benthamiana 叶片中，并在4天后取样，叶片中GUS的瞬时表达量增加了约5倍[7]。
| Cas No. | 2478-38-8 | SDF | |
| 别名 | 乙酰丁香酮 | ||
| Canonical SMILES | COC1=CC(=CC(OC)=C1O)C(C)=O | ||
| 分子式 | C10H12O4 | 分子量 | 196.2 |
| 溶解度 | DMSO : ≥ 100 mg/mL (509.68 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.0968 mL | 25.4842 mL | 50.9684 mL |
| 5 mM | 1.0194 mL | 5.0968 mL | 10.1937 mL |
| 10 mM | 0.5097 mL | 2.5484 mL | 5.0968 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet