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Ac-ANW-AMC

(Synonyms: Ac-Ala-Asn-Trp-AMC) 目录号 : GC42685

A fluorogenic substrate for the 20S immunoproteasome

Ac-ANW-AMC Chemical Structure

Cas No.:2357123-49-8

规格 价格 库存 购买数量
500μg
¥770.00
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1mg
¥1,473.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Ac-ANW-AMC is a fluorogenic substrate for the β5i/LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify the activity of the β5i/LMP7 subunit of the 20S immunoproteasome. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

Reference
[1]. Winter, M.B., La Greca, F., Arastu-Kapur, S., et al. Immunoproteasome functions explained by divergence in cleavage specificity and regulation. eLife 6:e27364, (2017).

Chemical Properties

Cas No. 2357123-49-8 SDF
别名 Ac-Ala-Asn-Trp-AMC
化学名 (S)-N1-((S)-3-(1H-indol-3-yl)-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopropan-2-yl)-2-((S)-2-acetamidopropanamido)succinamide
Canonical SMILES O=C1C=C(C)C2=C(C=C(NC([C@H](CC3=CNC4=C3C=CC=C4)NC([C@H](CC(N)=O)NC([C@H](C)NC(C)=O)=O)=O)=O)C=C2)O1
分子式 C30H32N6O7 分子量 588.6
溶解度 10mM in DMSO 储存条件 Store at -20°C, protect from light
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1 mM 1.6989 mL 8.4947 mL 16.9895 mL
5 mM 0.3398 mL 1.6989 mL 3.3979 mL
10 mM 0.1699 mL 0.8495 mL 1.6989 mL
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Research Update

Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates

Immune Netw 2022 Apr 15;22(3):e28.PMID:35799704DOI:10.4110/in.2022.22.e28.

The 26S proteasome irreversibly hydrolyzes polyubiquitylated substrates to maintain protein homeostasis; it also regulates immune responses by generating antigenic peptides. An alternative form of the 26S proteasome is the immunoproteasome, which contains substituted catalytic subunits (β1i/PSMB9, β2i/PSMB10, and β5i/PSMB8) instead of constitutively expressed counterparts (β1/PSMB6, β2/PSMB7, and β5/PSMB5). The immunoproteasome expands the peptide repertoire presented on MHC class I molecules. However, how its activity changes in this context is largely elusive, possibly due to the lack of a standardized methodology to evaluate its specific activity. Here, we describe an assay protocol that measures the immunoproteasome activity of whole-cell lysates using commercially available fluorogenic peptide substrates. Our results showed that the most accurate assessment of immunoproteasome activity could be achieved by combining β5i-targeting substrate Ac-ANW-AMC and immunoproteasome inhibitor ONX-0914. This simple and reliable protocol may contribute to future studies of immunoproteasomes and their pathophysiological roles during viral infection, inflammation, and tumorigenesis.