α-D-Glucose-1,6-bisphosphate (cyclohexyl ammonium salt hydrate)
(Synonyms: Α-D-葡萄糖-1,6-二磷酸酯四(环己基铵)盐水合物,D-Glucose-1,6-diphosphate Glc-1,6-P2) 目录号 : GC45201α-D-Glucose-1,6-biphosphate 是葡萄糖的衍生物，用于研究碳水化合物代谢，特别是在 α-磷酸葡萄糖变位酶、β-磷酸葡萄糖变位酶以及 α- 和 β-己糖变位酶的鉴定、区分和表征方面。
Cas No.:71662-13-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
α-D-Glucose-1,6-bisphosphate is a bis-phosphorylated derivative of α-D-glucose that has roles in carbohydrate metabolism.[1] It is the product of the reaction of glucose-1- or 6-phosphate with glucose-1,6-bisphosphate synthase (PGM2LI) in the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate.[2] It is also a cofactor for the bacterial enzyme phosphopentomutase.[3],[4] α-D-Glucose-1,6-bisphosphate has been used in the study of carbohydrate metabolism.
Reference：
[1]. Beitner, R. Regulation of carbohydrate metabolism by glucose 1,6-bisphosphate in extrahepatic tissues; comparison with fructose 2,6-bisphosphate. Int. J. Biochem. 22(6), 553-557 (1990).
[2]. Maliekal, P., Sokolova, T., Vertommen, D., et al. Molecular identification of mammalian phosphopentomutase and glucose-1,6-bisphosphate synthase, two members of the α-D-phosphohexomutase family. J. Biol. Chem. 282(44), 31844-31851 (2007).
[3]. Moustafa, H.M.A., Zaghloul, T.I., and Zhang, Y.-H.P. A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima. Anal. Biochem. 501, 75-81 (2016).
[4]. Panosian, T.D., Nannemann, D.P., Watkins, G.R., et al. Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle. J. Biol. Chem. 286(10), 8043-8054 (2011).
[1]. Beitner, R. Regulation of carbohydrate metabolism by glucose 1,6-bisphosphate in extrahepatic tissues; comparison with fructose 2,6-bisphosphate. Int. J. Biochem. 22(6), 553-557 (1990).
[2]. Maliekal, P., Sokolova, T., Vertommen, D., et al. Molecular identification of mammalian phosphopentomutase and glucose-1,6-bisphosphate synthase, two members of the α-D-phosphohexomutase family. J. Biol. Chem. 282(44), 31844-31851 (2007).
[3]. Moustafa, H.M.A., Zaghloul, T.I., and Zhang, Y.-H.P. A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima. Anal. Biochem. 501, 75-81 (2016).
[4]. Panosian, T.D., Nannemann, D.P., Watkins, G.R., et al. Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle. J. Biol. Chem. 286(10), 8043-8054 (2011).
| Cas No. | 71662-13-0 | SDF | |
| 别名 | Α-D-葡萄糖-1,6-二磷酸酯四(环己基铵)盐水合物,D-Glucose-1,6-diphosphate Glc-1,6-P2 | ||
| 化学名 | α-D-glucopyranose, 1,6-bis(dihydrogen phosphate), cyclohexanamine (1:4), hydrate | ||
| Canonical SMILES | O[C@@H]1[C@@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O[C@@H]1OP(O)(O)=O.NC2CCCCC2.NC3CCCCC3.NC4CCCCC4.NC5CCCCC5.O | ||
| 分子式 | C6H14O12P2•4C6H13N [XH2O] | 分子量 | 736.8 |
| 溶解度 | 10mg/mL in PBS, pH 7.2 | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3572 mL | 6.7861 mL | 13.5722 mL |
| 5 mM | 0.2714 mL | 1.3572 mL | 2.7144 mL |
| 10 mM | 0.1357 mL | 0.6786 mL | 1.3572 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。