9(S)-HODE-13C18
(Synonyms: (+)-α-Dimorphecolic Acid) 目录号 : GC46755
A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
9(S)-HODE-13C18 is intended for use as an internal standard for the quantification of 9(S)-HODE by GC- or LC-MS. 9(S)-HODE is a monohydroxy fatty acid formed from linoleic acid primarily by lipoxygenases with a 9-HpODE intermediate.1,2,3,4 It is an agonist of PPARγ, inducing transcription of AF-2 in a reporter assay when used at a concentration of 20 µM, and enhances the production of primary human chorionic gonadotropin (hCG) in trophoblasts.5 9(S)-HODE is also an agonist of transient receptor potential vanilloid 1 (TRPV1), TRP ankyrin 1 (TRPA1), and TRP melastatin 8 (TRPM8), increasing intracellular calcium levels in HEK293 cells (EC50s = 21, 31.9, and 43.6 µM, respectively, for the human receptors).6 It inhibits the migration of primary porcine aortic endothelial cells and phytohemagglutinin-induced migration of human mononuclear cells when used at concentrations ranging from 0.01 to 1 µM.7
1.Vick, B.A.Oxygenated fatty acids of the lipoxygenase pathwayLipid Metabolism in Plants167-191(1993) 2.Reinaud, O., Delaforge, M., Boucher, J.L., et al.Oxidative metabolism of linoleic acid by human leukocytesBiochem. Biophys. Res. Commun.161(2)883-891(1989) 3.Kim, E., Rundhaug, J.E., Benavides, F., et al.An antitumorigenic role for murine 8S-lipoxygenase in skin carcinogenesisOncogene24(7)1174-1187(2005) 4.Hamberg, M.Stereochemistry of oxygenation of linoleic acid catalyzed by prostaglandin-endoperoxide H synthase-2Arch. Biochem. Biophys.349(2)376-380(1998) 5.Schild, R.L., Schaiff, W.T., Carolson, M.G., et al.The activity of PPARγ in primary human trophoblasts is enhanced by oxidized lipidsJ. Clin. Endocrinol. Metab.87(3)1105-1110(2002) 6.De Petrocellis, L., Moriello, A.S., Imperatore, R., et al.A re-evaluation of 9-HODE activity at TRPV1 channels in comparison with anandamide: enantioselectivity and effects at other TRP channels and in sensory neuronsBr. J. Pharmacol.167(8)1643-1651(2012) 7.Pankonin, G., Mahmood, S.A., Kuhn, H., et al.Inhibition of cell migrations by the linoleic acid oxygenation product 9S-hydroxy 10E,12Z octadecadienoic acid (9-HODE)Biomed. Biochim. Acta.47(12)K17-21(1988)
| Cas No. | N/A | SDF | |
| 别名 | (+)-α-Dimorphecolic Acid | ||
| Canonical SMILES | O[13C]([13CH2][13CH2][13CH2][13CH2][13CH2][13CH2][13CH2][13C@H](O)/[13CH]=[13CH]/[13CH]=[13CH]\[13CH2][13CH2][13CH2][13CH2][13CH3])=O | ||
| 分子式 | [13C]18H32O3 | 分子量 | 314.3 |
| 溶解度 | DMF: 50 mg/ml,DMSO: 50 mg/ml,Ethanol: 50 mg/ml,PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1817 mL | 15.9084 mL | 31.8167 mL |
| 5 mM | 0.6363 mL | 3.1817 mL | 6.3633 mL |
| 10 mM | 0.3182 mL | 1.5908 mL | 3.1817 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。