8,12-iso-iPF2α-VI-d11

8,12-iso-isoprostane F_2α-VI-d₁₁ (8,12-iso-iPF_2α-VI-d₁₁) contains 11 deuterium atoms at the 16, 16', 17, 17', 18, 18', 19, 19', 20, 20, and 20 positions. It is intended for use as an internal standard for the quantification of 8,12-iso-iPF_2α-VI by GC- or LC-mass spectrometry. The relative abundance of deuterium atoms incorporated into each molecule has not been determined. certifies that less that 1% of the product has no deuterium incorporation (<1% d0). Isoprostanes are non-enzymatic, non-cyclooxygenase prostanoid products of peroxidative damage to membrane lipids.¹ Among the many isoprostane isomers, 8,12-iso-iPF_2α-VI has been demonstrated to be one of the predominant isomers formed and is also present in urine as one of the major isoprostane products.² 8,12-iso-iPF_2α-VI-d₁₁ is a deuterated internal standard for use in isoprostane quantitation by mass spec modalities. The compound is diastereomeric at C-5, but is otherwise an optically active, single enantiomer.

1.Diaz, M.N., Frei, B., Vita, J.A., et al.Antoixidants and atherosclerotic heart diseaseNew England Journal of Medicine337408-416(1997) 2.Lawson, J.A., Li, H., Rokach, J., et al.Identification of two major F2 isoprostanes, 8,12-iso- and 5-epi-8,12-iso-isoprostane F2α-VI, in human urineThe Journal of Biological Chemisty27329295-29301(1998)