6-OAU
(Synonyms: GTPL5846) 目录号 : GC11350
An agonist for GPR84
Cas No.:83797-69-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
6-OAU is a synthetic agonist for GPR84.
GPR84, a Gαi protein-coupled receptor, is activated by medium-chain free fatty acids, but only at micromolar concentrations. GPR84 is also a putative receptor for medium-chain fatty acids. GPR84 is expressed in leukocytes and its expression can be further induced by LPS.
In vitro: A previous study identified 6-OAU as a surrogate agonist for GPR84. It was found that 6-OAU and potential ligands were able to stimulate [(35)S]GTP binding and accumulate phosphoinositides in a GPR84-dependent manner. Moreover, 6-OAU could internalize GPR84-EGFP from the cell surface. In addition, results showed that both 6-OAU and the potential ligands could elicite chemotaxis of human polymorphonuclear leukocytes (PMNs) and macrophages and amplify LPS-stimulated production of the proinflammatory cytokine IL-8 from PMNs and TNFα from macrophages [1].
In vivo: Animal study found that the iv injection of 6-OAU was able to raise the blood CXCL1 level in rats. In addition, the inoculation of 6-OAU into the rat air pouch could accumulate PMNs and macrophages in the site [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Suzuki, M. ,Takaishi, S.,Nagasaki, M., et al. Medium-chain fatty acid-sensing receptor, GPR84, is a proinflammatory receptor. The Journal of Biological Chemisty 288(15), 10684-10691 (2013).
| Cas No. | 83797-69-7 | SDF | |
| 别名 | GTPL5846 | ||
| 化学名 | 6-(octylamino)-2,4(1H,3H)-pyrimidinedione | ||
| Canonical SMILES | O=C1NC(NCCCCCCCC)=CC(N1)=O | ||
| 分子式 | C12H21N3O2 | 分子量 | 239.3 |
| 溶解度 | ≤0.25mg/ml in DMSO;0.25mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.1789 mL | 20.8943 mL | 41.7885 mL |
| 5 mM | 0.8358 mL | 4.1789 mL | 8.3577 mL |
| 10 mM | 0.4179 mL | 2.0894 mL | 4.1789 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。