5-Me-CTP,100mM Sodium Solution

5-Methyl-CTP (5-Me-CTP) is a modified nucleoside triphosphate commonly used to replace naturally occurring CTP during in vitro mRNA synthesis, increasing mRNA stability and protein expression while reducing innate immunity Interaction of receptors with immune cells[1]. 5-Methylcytosine is the only naturally occurring modified base in animal DNA, and it is found in mRNA, rRNA and tRNA of various representative organisms[2]. 5-Methylcytosine fifth C-atom methylation is a reversible epigenetic modification that, when present near gene regulatory regions, can regulate the binding of transcription factors or induce the binding of specific 5 mC-binding proteins, resulting in co-repression The complex is recruited to methylated target promoters[3]. RNA 5-Me-CTP modification affects the fate of the modified RNA molecule, including promotion of mRNA stability, splicing and nucleoplasmic transport, DNA damage repair; mRNA stability; cell tolerance, proliferation and migration; stem cell development, Differentiation and reprogramming[3]. In addition, the distribution of 5-Me-CTP varies among cell types, and m5C modification at specific positions of mRNA exhibits different regulatory activities[4].

References:
[1]. Muthian Shanmugasundaram, et al. An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates. Nucleosides Nucleotides Nucleic Acids. 2016 Jul 2;35(7):356-62.
[2]. D. Riggs, Peter A. Jones. 5-Methylcytosine, Gene Regulation, and Cancer. Science, 1981 - science.org.
[3]. Achim Breiling.Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond. 2015 Jul 21;8:24. doi: 10.1186/s13072-015-0016-6. eCollection 2015.
[4]. Hang Song, et al. Biological roles of RNA m5C modification and its implications in Cancer immunotherapy. 2022 Apr 1;10(1):15. doi: 10.1186/s40364-022-00362-8.

5-Methyl-CTP(5-Me-CTP)是一种修饰的核苷三磷酸,通常用于在体外mRNA合成过程中替代天然存在的CTP,增加mRNA的稳定性和蛋白表达,同时减少天然免疫受体与免疫细胞的相互作用[1]。5-Methylcytosine是动物DNA中唯一天然存在的修饰碱基,在各种代表性生物的mRNA、rRNA和tRNA中均有发现[2]。5-Methylcytosine第五位C原子甲基化是一种可逆的表观遗传修饰,当存在于基因调控区域附近时能够调节转录因子的结合或诱导特异性5 mC结合蛋白的结合,从而导致共阻遏物复合物募集到甲基化靶启动子[3]。RNA 5-Me-CTP修饰会影响修饰后的RNA分子的命运,包括促进mRNA稳定性、剪接和核质运输、DNA损伤修复；mRNA 稳定性；细胞耐受性、增殖和迁移；干细胞的发育、分化和重编程[3]。此外,5-Me-CTP的分布因细胞类型而异,在mRNA的特定位置上的m5C修饰表现出不同的调控活性[4]。