2'-Deoxycytidine-5'-triphosphate (sodium salt)
(Synonyms: 2'-脱氧胞苷-5'-三磷酸三钠盐,dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt) 目录号 : GC48440
A nucleoside triphosphate
Cas No.:109909-44-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2'-Deoxycytidine-5'-triphosphate (dCTP) is a nucleoside triphosphate.1 It has commonly been used in PCR reaction mixtures as a substrate for DNA synthesis by DNA polymerases.
1.Mohsen, M.G., Ji, D., and Kool, E.T.Polymerase synthesis of four-base DNA from two stable dimeric nucleotidesNucleic Acids Res.47(18)9495-9501(2019)
| Cas No. | 109909-44-6 | SDF | |
| 别名 | 2'-脱氧胞苷-5'-三磷酸三钠盐,dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt | ||
| Canonical SMILES | O[C@@H]1[C@@H](COP(OP(OP(O)([O-])=O)([O-])=O)([O-])=O)O[C@](N2C=CC(N)=NC2=O)([H])C1.[Na+].[Na+].[Na+] | ||
| 分子式 | C9H13N3O13P3•3Na | 分子量 | 533.1 |
| 溶解度 | Water: 10 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8758 mL | 9.3791 mL | 18.7582 mL |
| 5 mM | 0.3752 mL | 1.8758 mL | 3.7516 mL |
| 10 mM | 0.1876 mL | 0.9379 mL | 1.8758 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Gram-scale chemical synthesis of 2'-deoxynucleoside-5'-o-triphosphates
Curr Protoc Nucleic Acid Chem 2012 Jun;Chapter 13:Unit13.10.PMID:22700336DOI:10.1002/0471142700.nc1310s49.
A simple, straightforward, reliable, and efficient method for the chemical synthesis of sodium salt of 2'-deoxynucleoside-5'-O-triphosphates (dNTPs), starting from the corresponding nucleoside, is described. This improved "one-pot, three-step" synthetic strategy involves the monophosphorylation of nucleoside, followed by reaction with tributylammonium pyrophosphate and hydrolysis of the resulting cyclic intermediate to provide the corresponding dNTP in good yields (65% to 70%). It is noteworthy that the protocol holds good for both the purine deoxynucleotides, such as 2'-deoxyguanosine-5'-O-triphosphate (dGTP) and 2'-deoxyadenosine-5'-O-triphosphate (dATP), and pyrimidine deoxynucleotides, such as 2'-deoxycytidine-5'-O-triphosphate (dCTP), thymidine-5'-O-triphosphate (TTP), and 2'-deoxyuridine-5'-O-triphosphate (dUTP).