(±)13-HpODE
(Synonyms: 13-Hydroperoxylinoleic acid; Linoleic acid 13-hydroperoxide) 目录号 : GC40355Hydroperoxide mixture derived from linoleic acid
Cas No.:23017-93-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
(±)13-HpODE is a racemic mixture of hydroperoxides derived non-enzymatically from linoleic acid through the action of reactive oxygen species. Lipid hydroperoxides, including 13-HpODE, have limited direct biological actions, such as the activation of lipoxygenases. [1] Normally, (±)13-HpODE is rapidly reduced to (±)13-HODE, a compound which exhibits many biological activities. [2] Importantly, 13-HpODE can also be metabolized to unsaturated aldehydes that can, in turn, covalently bind to DNA, amino acids, and proteins.[3][4][5]
Reference:
[1]. Jones, G.D., Russell, L., Darley-Usmar, V.M., et al. Role of lipid hydroperoxides in the activation of 15-lipoxygenase. Biochemistry 35, 7197-7203 (1996).
[2]. Kühn, H. Biosynthesis, metabolization and biological importance of the primary 15-lipoxygenase metabolites 15-hydro(pero)xy-5Z,8Z,11Z,13E-eicosatetraenoic acid and 13-hydro(pero)xy-9Z,11E-octadecadienoic acid. Progress in Lipid Research 35, 203-226 (1996).
[3]. Williams, M.V., Wishnok, J.S., and Tannenbaum, S.R. Covalent adducts arising from the decomposition products lipid hydroperoxides in the presence of cytochrome C. Chemical Research in Toxicology 20(5), 767-775 (2007).
[4]. Iwahashi, H., Nishizaki, K., and Takagi, I. Cytochrome c catalyzes the formation of pentyl radical and octanoic acid radical from linoleic acid hydroperoxide. Biochemistry Journal 361, 57-66 (2002).
[5]. Kawai, Y., Kato, Y., Fujii, H., et al. Immunochemical detection of a novel lysine adduct using an antibody to linoleic acid hydroperoxide-modifies protein. Journal of Lipid Research 44, 1124-1131 (2003).
| Cas No. | 23017-93-8 | SDF | |
| 别名 | 13-Hydroperoxylinoleic acid; Linoleic acid 13-hydroperoxide | ||
| 化学名 | (±)13-hydroperoxy-9Z,11E-octadecadienoic acid | ||
| Canonical SMILES | CCCCCC(OO)/C=C/C=C\CCCCCCCC(O)=O | ||
| 分子式 | C18H32O4 | 分子量 | 312.4 |
| 溶解度 | 50 mg/ml in DMF, 50 mg/ml in DMSO, 50 mg/ml in ethanol | 储存条件 | Store at -80°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.201 mL | 16.0051 mL | 32.0102 mL |
| 5 mM | 0.6402 mL | 3.201 mL | 6.402 mL |
| 10 mM | 0.3201 mL | 1.6005 mL | 3.201 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。