1-Stearoyl-2-Arachidonoyl-sn-glycero-3-PE
目录号 : GC42043A phospholipid
Sample solution is provided at 25 µL, 10mM.
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1-Stearoyl-2-arachidonoyl-sn-glycero-3-PE is a naturally-occurring phospholipid that is formed via the phosphatidylserine decarboxylation pathway in mammalian cells.[1] It has been used in the generation of giant unilamellar vesicles for use in the study of biological membranes.[2]
Reference:
[1]. Bleijerveld, O.B., Brouwers, J.F., Vaandrager, A.B., et al. The CDP-ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine molecular species. J. Biol. Chem. 282(39), 28362-28372 (2007).
[2]. Billerit, C., Jeffries, G.D.M., Orwar, O., et al. Formation of giant unilamellar vesicles from spin-coated lipid films by localized IR heating. Soft Matter 8(42), 10823-10826 (2012).
| Cas No. | 61216-62-4 | SDF | |
| 别名 | SAPE | ||
| 化学名 | 5Z,8Z,11Z,14Z-eicosatetraenoic acid, (1R)-1-[[[(2-aminoethoxy)hydroxyphosphinyl]oxy]methyl]-2-[(1-oxooctadecyl)oxy]ethyl ester | ||
| Canonical SMILES | O=C(CCCCCCCCCCCCCCCCC)OC[C@@H](OC(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)=O)COP(OCC[NH3+])([O-])=O | ||
| 分子式 | C43H78NO8P | 分子量 | 768.1 |
| 溶解度 | Ethanol:PBS (pH 7.2)(1:2): 1 mg/ml Methanol: 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。