1,2-Dimyristoyl-sn-glycero-3-PC
(Synonyms: 二肉豆蔻酰磷脂酰胆碱（DMPC）,DMPC; Dimyristoyl phosphatidylcholine) 目录号 : GC41803
1,2-Dimyristoyl-sn-glycero-3-PC是一种合成磷脂。
Cas No.:18194-24-6
Sample solution is provided at 25 µL, 10mM.
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1,2-Dimyristoyl-sn-glycero-3-PC is a synthetic phospholipid[1]. 1,2-Dimyristoyl-sn-glycero-3-PC is usually used to construct liposomes for the study of lipid monolayer and bilayer structures and drug delivery systems[2][3]. 1,2-Dimyristoyl-sn-glycero-3-PC can also be used in metabolism-related research[4].
In vitro, 1,2-Dimyristoyl-sn-glycero-3-PC (200μg/mL; 24h) significantly enhanced PVP-SPIONs uptake in PC-12 cells[5]. 1,2-Dimyristoyl-sn-glycero-3-PC (0.08-250μM; 72h) increases Carmofur stability and antiproliferative effect in C6 glioma cells[6].
In vivo, 1,2-Dimyristoyl-sn-glycero-3-PC (2-2.5mg/day/mouse in drinking water; 8 weeks) significantly reduced aortic sinus lesion area and en face whole aorta lesion area in 6-month-old male apoE-null mice[7].
References:
[1] Fountain MW, Ganjam VK, Schultz RD. Liposome carrier vehicle for triiodothryonine. Clin Exp Pharmacol Physiol. 1982;9(1):101-105.
[2] Budziak I, Arczewska M, Sachadyn-Król M, et al. Effect of polyols on the DMPC lipid monolayers and bilayers. Biochim Biophys Acta Biomembr. 2018;1860(11):2166-2174.
[3] Marcos X, Sixto-López Y, Pérez-Casas S, Correa-Basurto J. Computational study of DMPC liposomes loaded with the N-(2-Hydroxyphenyl)-2-propylpentanamide (HO-AAVPA) and determination of its antiproliferative activity in vitro in NIH-3T3 cells. J Biomol Struct Dyn. 2022;40(21):11448-11459.
[4] Du K, Sun L, Luo Z, et al. Reduced DMPC and PMPC in lung surfactant promote SARS-CoV-2 infection in obesity. Metabolism. 2022;131:155181.
[5] Han D, Zhang B, Dong J, et al. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine promotes the adhesion of nanoparticles to bio-membranes and transport in rat brain. RSC Adv. 2021;11(56):35455-35462.
[6] Domracheva I, Muhamadejev R, Petrova M, et al. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) increases Carmofur stability and in vitro antiproliferative effect. Toxicol Rep. 2015;2:377-383.
[7] Navab M, Hama S, Hough G, Fogelman AM. Oral synthetic phospholipid (DMPC) raises high-density lipoprotein cholesterol levels, improves high-density lipoprotein function, and markedly reduces atherosclerosis in apolipoprotein E-null mice. Circulation. 2003;108(14):1735-1739.
1,2-Dimyristoyl-sn-glycero-3-PC是一种合成磷脂[1]。1,2-Dimyristoyl-sn-glycero-3-PC通常用于构建脂质体以研究脂质单层和双层结构以及药物递送系统[2][3]。1,2-Dimyristoyl-sn-glycero-3-PC还可用于代谢相关研究[4]。
在体外实验中，1,2-Dimyristoyl-sn-glycero-3-PC（200μg/mL；24小时）显著增强了PC-12细胞对PVP-SPIONs的摄取[5]。1,2-Dimyristoyl-sn-glycero-3-PC (0.08-250μM；72小时）在C6胶质瘤细胞中提高卡莫福尔的稳定性，并增强抗增殖效果[6]。
在体内实验中，1,2-Dimyristoyl-sn-glycero-3-PC（2-2.5mg/day/mouse；通过饮用水给药；持续8周）显著减少了6个月大雄性apoE基因敲除小鼠的主动脉窦病变面积和全主动脉病变面积[7]。
| Cell experiment [1]: | |
Cell lines | C6 glioma cells |
Preparation Method | 1,2-Dimyristoyl-sn-glycero-3-PC vesicles were prepared as follows: Carmofur (26mg, 0.1mmol) and 1,2-Dimyristoyl-sn-glycero-3-PC (0.334g, 0.5mmol) were dissolved in 25ml of chloroform and evaporated on rotatory evaporator under reduced pressure with slow rotation without any heating. Then additional portion of chloroform was added and the procedure was repeated for 5 times. C6 glioma cells were grown in standard medium Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum. About 1-2×104cells/well were placed in 96-well plates immediately after 1,2-Dimyristoyl-sn-glycero-3-PC vesicles (250, 50, 10, 2, 0.4 and 0.08μM) were added to the wells. The plates were incubated for 72h, at 37℃, 5% CO2. The number of surviving cells was determined using MTT assay. The control cells were cultured on separate plate and an appropriate volume of control buffer was added to control cells. Each assay was conducted in triplicate. |
Reaction Conditions | 0.08-250μM; 72h |
Applications | 1,2-Dimyristoyl-sn-glycero-3-PC increases Carmofur stability and antiproliferative effect in C6 glioma cells. |
| Animal experiment [2]: | |
Animal models | Male and female apoE-null mice |
Preparation Method | Male and female apoE-null mice were maintained on a chow diet. The 1,2-Dimyristoyl-sn-glycero-3-PC, obtained in the form of a fine powder, readily resulted in a uniform suspension when mixed in water for 1mg/mL. The suspension was added to 50-mL graduated polypropylene tubes fitted with standard rubber stoppers and stainless steel tubes of a type used routinely in mouse water bottles. 1mg/mL 1,2-Dimyristoyl-sn-glycero-3-PC in the drinking water was given to mice for 8 weeks. The tubes were observed daily, and the lipids were found by inspection to remain in suspension. The contents of the tubes were changed every other day. The mice drank 2 to 2.5mL/d per mouse, and there was no significant difference in the amount of suspension consumed between the groups. Aortic lesions were scored by histopathology analysis. |
Dosage form | 2-2.5mg/day/mouse in drinking water; 8 weeks |
Applications | 1,2-Dimyristoyl-sn-glycero-3-PC significantly reduced aortic sinus lesion area and en face whole aorta lesion area in 6-month-old male apoE-null mice. |
References: | |
| Cas No. | 18194-24-6 | SDF | |
| 别名 | 二肉豆蔻酰磷脂酰胆碱（DMPC）,DMPC; Dimyristoyl phosphatidylcholine | ||
| Canonical SMILES | O=C(CCCCCCCCCCCCC)OC[C@@H](OC(CCCCCCCCCCCCC)=O)COP(OCC[N+](C)(C)C)([O-])=O | ||
| 分子式 | C36H72NO8P | 分子量 | 677.9 |
| 溶解度 | Ethanol: 25 mg/ml | 储存条件 | Store at 2-8°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4751 mL | 7.3757 mL | 14.7514 mL |
| 5 mM | 295 μL | 1.4751 mL | 2.9503 mL |
| 10 mM | 147.5 μL | 737.6 μL | 1.4751 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet