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(Z-Ala-Ala-Ala-Ala)2Rh110

(Synonyms: (Z-Ala-Ala-Ala-Ala)2Rhodamine110|bis-(CBZ-L-alanyl-L-arginine amide) Rhodamine 110) 目录号 : GC11847

A fluorogenic elastase substrate

(Z-Ala-Ala-Ala-Ala)2Rh110 Chemical Structure

Cas No.:149695-85-2

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1mg
¥606.00
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5mg
¥2,725.00
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10mg
¥4,542.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

(Z-Ala-Ala-Ala-Ala)2Rh110 is a fluorogenic elastase substrate.

Elastase can break down elastin that is an elastic fibre that, together with collagen and can determine the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A of E. coli and other Gram-negative bacteria. In addition, elastase has the critical immunological role of breaking down Shigella virulence factors.

In vitro: (Z-Ala-Ala-Ala-Ala)2Rh110 has been identified as a very sensitive fluorogenic elastase substrate. The colorless and nonfluorescent Z-Ala-Ala-Ala-Ala)2Rh110 was found to be selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which exhibited excellent spectral properties matching the optimal detection window of most fluorescence instruments. After cleavage, Abs(max) is at 497 nm and Em(max) is at 520 nm for rhodamine 110 [1].

In vivo: Currently, there is no animal in vivo data reported.

Clinical trial: So far, no clinical study has been conducted.

Reference:
[1] A.  F. Johnson, M. D. Struthers, K. B. Pierson, et al. Nonisotopic DNA detection system employing elastase and a fluorogenic rhodamine substrate. Analytical Chemistry 65, 2352-2359 (1993).

Chemical Properties

Cas No. 149695-85-2 SDF
别名 (Z-Ala-Ala-Ala-Ala)2Rhodamine110|bis-(CBZ-L-alanyl-L-arginine amide) Rhodamine 110
化学名 N-[(phenylmethoxy)carbonyl]-L-alanyl-L-alanyl-L-alanyl-N-[3-oxo-6’-[[N-[N-[N-[N-[(phenylmethoxy)carbonyl]-L-alanyl]-L-alanyl]-L-alanyl]-L-alanyl]amino]spiro[isobenzofuran-1(3H),9’-[9H
Canonical SMILES O=C(O1)C2=CC=CC=C2C31C4=C(C=C(NC(C(C)NC(C(C)NC(C(C)NC(C(C)NC(OCC5=CC=CC=C5)=O)=O)=O)=O)=O)C=C4)OC6=C3C=CC(NC(C(C)NC(C(C)NC(C(C)NC(C(C)NC(OCC7=CC=CC=C7)=O)=O)=O)=O)=O)=C6
分子式 C60H66N10O15 分子量 1167.2
溶解度 ≤2mg/ml in DMSO;2mg/ml in dimethyl formamide 储存条件 Store at -20°C,protect from light
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 0.8568 mL 4.2838 mL 8.5675 mL
5 mM 0.1714 mL 0.8568 mL 1.7135 mL
10 mM 0.0857 mL 0.4284 mL 0.8568 mL
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