Yeast Extract
(Synonyms: 酵母提取物) 目录号 : GC17277
微生物培养基
Cas No.:8013-01-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cas No. | 8013-01-2 | SDF | |
| 别名 | 酵母提取物 | ||
| 化学名 | 2-(11H-benzo[a]fluoren-11-yl)acetic acid | ||
| Canonical SMILES | O=C(O)CC1C2=CC=CC=C2C3=C1C4=CC=CC=C4C=C3 | ||
| 分子式 | C19H14O2 | 分子量 | 274.31 |
| 溶解度 | ≥ 27.4 mg/mL in Water | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6455 mL | 18.2276 mL | 36.4551 mL |
| 5 mM | 0.7291 mL | 3.6455 mL | 7.291 mL |
| 10 mM | 0.3646 mL | 1.8228 mL | 3.6455 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Production and characterization of yeast extracts produced by Saccharomyces cerevisiae, Saccharomyces boulardii and Kluyveromyces marxianus
Prep Biochem Biotechnol 2022;52(6):657-667.34632953 10.1080/10826068.2021.1983833
In recent years, prejudice in society against monosodium glutamate (MSG) has directed food manufacturers to alternative sources. Yeast extracts are considered as "natural" due to the production process and stand out due to their nutritional properties as well as giving a flavor similar to MSG. In this study, chemical, functional and flavor properties of Yeast Extract powders produced from Saccharomyces cerevisiae TGM10, Saccharomyces boulardii S11 and Kluyveromyces marxianus TGM66 were evaluated. Results revealed that the most protein-rich sample was S. cerevisiae TGM10 extract (69.17%), followed by S. boulardii S11 (66.16%) and K. marxianus TGM66 (62.42%) extracts, respectively and S. cerevisiae TGM10 extract was also the richest Yeast Extract for essential amino acids. Additionally, flavor-enhancing amino acids such as glutamic acid, aspartic acid, alanine and glycine were dominant in S. cerevisiae TGM10 extract (47.41 g/100 g protein). Sensorial evaluation of yeast extracts demonstrated that salty taste, umami taste and meaty flavor scores of yeast extracts were lower than MSG whereas for fruity flavor, yeast extracts had the highest scores. These findings revealed the potential of three yeast strains to produce yeast extracts in order to increase the nutritional value and flavor of foods.
Catalytic activity of Yeast Extract in biofuel cell
J Biosci Bioeng 2012 Nov;114(5):521-5.22795667 10.1016/j.jbiosc.2012.05.021
It was revealed that the dry powder Yeast Extract (YE) has the ability to act as a biocatalyst as well as a mediator in a biofuel cell. The Yeast Extract, from Nihon Pharmaceutical Co., Ltd., was used as a biocatalyst in an open air cathode biofuel cell containing phosphate buffer for glucose oxidation. The anode medium with only the YE showed an immediate activity by producing a current and delivering power depending on its concentration. By adding glucose to the anode medium, the anode potential decreased with time to -0.2 V vs. normal hydrogen electrode (NHE), and produced a higher power compared to that without glucose. The biofuel cell produced an open circuit voltage (OCV) as high as 1 V.
Minor element composition of Yeast Extract
J Bacteriol 1962 Oct;84(4):869-70.13949853 PMC277973
Trophic effect of a methanol Yeast Extract on 3T3 fibroblast cells
J Cosmet Sci 2014 Nov-Dec;65(6):389-401.25898765
With regard to the increase of human life expectancy, interest for topical treatments aimed to counteract skin aging is still growing. Hence, research for bioactive compounds able to stimulate skin fibroblast activity is an attractive topic. Having previously described the effects of a new methanol Yeast Extract on growth and metabolic activity of Saccharomyces cerevisiae, we studied its effects on 3T3 fibroblasts to evaluate its potential antiaging property. This investigation demonstrates that this extract increases proliferation as well as migration of 3T3 cells and decreases their entrance in senescence and apoptosis phases. Altogether, these results open new perspectives for the formulation of innovative antiaging topical treatments.
Preparation of a 纬-glutamylcysteine-enriched Yeast Extract from a newly developed GSH2-deficient strain
J Biosci Bioeng 2013 Jan;115(1):50-4.22986308 10.1016/j.jbiosc.2012.08.009
Gamma-glutamylcysteine (纬-GC), the precursor of glutathione (GSH), may have significant health benefits as a dietary supplement, but there are few cost-effective methods available for its large-scale production. We developed an efficient method for producing 纬-GC in a mutant yeast strain using a three-step breeding procedure and a unique cultivation process. In the first breeding step, we prepared a glutathione synthetase (GSH2)-deficient yeast mutant. In the second step, selenate (SeO(4)(2-)) sensitivity was introduced by crossing the GSH2-deficient mutant with a strain harboring the met30 mutation. In the final step, pantothenic acid auxotrophy was introduced by ethyl methanesulfonate mutagenesis. The isolated strain displayed significantly enhanced cellular 纬-GC when cultivated in synthetic medium without pantothenic acid, reaching a maximum level of 4.39% of dry cell weight. Using this strain, we were able to prepare a Yeast Extract containing approximately 13% 纬-GC (w/w), which is markedly higher than the reported value (0.3%) of commercially available yeast extracts. The present method may facilitate large-scale 纬-GC production for investigating the nutritive value and other benefits of dietary 纬-GC.