Tin protoporphyrin IX dichloride
(Synonyms: 锡原卟啉IX; SnPPIX; Stannous protoporphyrin IX) 目录号 : GC10800An inhibitor of heme oxygenase
Cas No.:14325-05-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Heme oxygenase (HO) converts protoheme to biliverdin, which in turn is enzymatically metabolized to bilirubin. While HO-2 is constitutively expressed, HO-1 can be induced by its heme substrate as well as by heavy metals, oxidizing agents, and other environmental stresses.[1],[2],[3] Tin protoporphyrin IX (SnPPIX) is a synthetic heme analog that selectively inhibits HO-1 (Ki = 11 nM) over HO-2 (IC50 = 7.5 µM).[4],[5] It also weakly inhibits endothelial nitric oxide synthase and soluble guanylyl cyclase (IC50s = 35 and 30 nM, respectively).[5] SnPPIX prevents hyperbilirubinemia in neonates by blocking HO-1 activity that increases postnatally.[4] It is rapidly cleared from plasma and persists in certain tissues, including kidney, liver, and spleen.[6] SnPPIX is commonly used as a tool to study the role of HO-1 activity in cells and in animals.[7[,[8]
Reference:
[1]. Kim, A.N., Jeon, W.K., Lee, J.J., et al. Up-regulation of heme oxygenase-1 expression through CaMKII-ERK1/2-Nrf2 signaling mediates the anti-inflammatory effect of bisdemethoxycurcumin in LPS-stimulated macrophages. Free Radical Biology & Medicine 49(3), 323-331 (2010).
[2]. Cable, E.E., Gildemeister, O.S., Pepe, J.A., et al. Mechanism of induction of heme oxygenase by metalloporphyrins in primary chick embryo liver cells: Evidence against a stress-mediated response. Molecular and Cellular Biochemistry 169, 13-20 (1997).
[3]. Surh, Y.J., Kundu, J.K., Li, M.H., et al. Role of Nrf2-mediated heme oxygenase-1 upregulation in adaptive survival response to nitrosative stress. Archives of Pharmacal Research 32(8), 1163-1176 (2009).
[4]. Drummond, G.S., and Kappas, A. Prevention of neonatal hyperbilirubinemia by tin protoporphyrin IX, a potent competitive inhibitor of heme oxidation. Proceedings of the National Academy of Sciences of the United States of America 78(10), 6466-6470 (1981).
[5]. Zakhary, R., Gaine, S.P., Dinerman, J.L., et al. Heme oxygenase 2: Endothelial and neuronal localization and role in endothelium-dependent relaxation. Proceedings of the National Academy of Sciences of the United States of America 93(2), 795-798 (1996).
[6]. Anderson, K.E., Simionatto, C.S., Drummond, G.S., et al. Tissue distribution and disposition of tin-protoporphyrin, a potent competitive inhibitor of heme oxygenase. Journal of Pharmacology and Experimental Therapeutics 228(2), 327-333 (1984).
[7]. Byun, S.J., Son, Y., and Pae, H.O. Cytoprotective effect of β-lapachone by inducing heme oxygenase-1 expression and AMP-activated protein kinase activation in human endothelial cells. Eur.Rev.Med.Pharmacol.Sci. 18(7), 949-958 (2014).
[8]. Zhang, M., Wang, S., Mao, L., et al. Omega-3 fatty acids protect the brain against ischemic injury by activating Nrf2 and upregulating heme oxygenase 1. Journal of Neuroscience 34(5), 1903-1915 (2014).
Cas No. | 14325-05-4 | SDF | |
别名 | 锡原卟啉IX; SnPPIX; Stannous protoporphyrin IX | ||
化学名 | (OC-6-13)-dichloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-κN21,κN22,κN23,κN24]-stannate(2-), dihydrogen | ||
Canonical SMILES | C=CC1=C(/C2=C([H])/C(C(C)=C/3CCC([O-])=O)=NC3=C([H])/C4=N/C(C(C)=C4CCC([O-])=O)=C([H])\C5=C(C(C)=C([N-]5)/C([H])=C1\[N-]2)C=C)C.Cl.Cl.[Sn+4] | ||
分子式 | C34H32Cl2N4O4Sn | 分子量 | 750.25 |
溶解度 | 0.5 mg/ml in DMSO, 1 mg/ml in DMF,0.1 M NaOH : 14.29 mg/mL (19.05 mM; ultrasonic and adjust pH to 12 with NaOH) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.3329 mL | 6.6644 mL | 13.3289 mL |
5 mM | 0.2666 mL | 1.3329 mL | 2.6658 mL |
10 mM | 0.1333 mL | 0.6664 mL | 1.3329 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。