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Thionin acetate (Thionine acetate) Sale

(Synonyms: 硫堇(劳氏紫),Thionine acetate) 目录号 : GC33590

醋酸硫素(Thionine acetate)(Thionine acetate)是一种异色性阳离子组织学染料,广泛用于生物染色。

Thionin acetate (Thionine acetate) Chemical Structure

Cas No.:78338-22-4

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1g
¥625.00
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产品描述

Thionin (acetate) is a metachromic cationic histology dye used in biological staining widely.

Chemical Properties

Cas No. 78338-22-4 SDF
别名 硫堇(劳氏紫),Thionine acetate
Canonical SMILES NC1=CC2=[S+]C3=C(C=CC(N)=C3)N=C2C=C1.CC([O-])=O
分子式 C14H13N3O2S 分子量 287.34
溶解度 DMSO: 41.67 mg/mL (145.02 mM) 储存条件 Store at -20°C
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1 mg 5 mg 10 mg
1 mM 3.4802 mL 17.401 mL 34.802 mL
5 mM 0.696 mL 3.4802 mL 6.9604 mL
10 mM 0.348 mL 1.7401 mL 3.4802 mL
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Research Update

The Lysozyme Inhibitor Thionine acetate Is Also an Inhibitor of the Soluble Lytic Transglycosylase Slt35 from Escherichia coli

Molecules 2021 Jul 9;26(14):4189.PMID:34299465DOI:10.3390/molecules26144189.

Lytic transglycosylases such as Slt35 from E. coli are enzymes involved in bacterial cell wall remodelling and recycling, which represent potential targets for novel antibacterial agents. Here, we investigated a series of known glycosidase inhibitors for their ability to inhibit Slt35. While glycosidase inhibitors such as 1-deoxynojirimycin, castanospermine, thiamet G and miglitol had no effect, the phenothiazinium dye Thionine acetate was found to be a weak inhibitor. IC50 values and binding constants for Thionine acetate were similar for Slt35 and the hen egg white lysozyme. Molecular docking simulations suggest that thionine binds to the active site of both Slt35 and lysozyme, although it does not make direct interactions with the side-chain of the catalytic Asp and Glu residues as might be expected based on other inhibitors. Thionine acetate also increased the potency of the beta-lactam antibiotic ampicillin against a laboratory strain of E. coli.

The use of filter paper discs impregnated with Thionin acetate, basic fuchsin and thionin blue in the identification of Brucella species

Onderstepoort J Vet Res 1990 Sep;57(3):197-9.PMID:2234867doi

Filter paper discs impregnated with solutions containing 0.25, 0.5 and 1 mg/milliliter of Thionin acetate, 0.75 and 1.5 mg/milliliter of basic fuchsin and 0.5 mg/milliliter of thionin blue were used in the typing of Brucella species. All the strains used reacted as expected, proving this new technique to be reliable in the identification of Brucella species. The method is less expensive and the results easier to interpret than those obtained with methods previously used.

Color-variable dual-dyed photodynamic antimicrobial polyethylene terephthalate (PET)/cotton blended fabrics

Photochem Photobiol Sci 2023 Mar 10;1-18.PMID:36894800DOI:10.1007/s43630-023-00398-1.

The urgent demand for scalable, potent, color variable, and comfortable antimicrobial textiles as personal protection equipment (PPE) to help reduce infection transmission in hospitals and healthcare facilities has significantly increased since the start of the COVID-19 pandemic. Here, we explored photodynamic antimicrobial polyethylene terephthalate/cotton (TC) blended fabrics comprised of photosensitizer-conjugated cotton fibers and polyethylene terephthalate (PET) fibers dyed with disperse dyes. A small library of TC blended fabrics was constructed wherein the PET fibers were embedded with traditional disperse dyes dominating the fabric color, thereby enabling variable color expression, while the cotton fibers were covalently coupled with the photosensitizer Thionine acetate as the microbicidal agent. Physical (SEM, CLSM, TGA, XPS and mechanical strength) and colorimetric (K/S and CIELab values) characterization methods were employed to investigate the resultant fabrics, and photooxidation studies with DPBF demonstrated the ability of these materials to generate reactive oxygen species (i.e., singlet oxygen) upon visible light illumination. The best results demonstrated a photodynamic inactivation of 99.985% (~ 3.82 log unit reduction, P = 0.0021) against Gram-positive S. aureus, and detection limit inactivation (99.99%, 4 log unit reduction, P ≤ 0.0001) against Gram-negative E. coli upon illumination with visible light (60 min; ~ 300 mW/cm2; λ ≥ 420 nm). Enveloped human coronavirus 229E showed a photodynamic susceptibility of ~ 99.99% inactivation after 60 min illumination (400-700 nm, 65 ± 5 mW/cm2). The presence of the disperse dyes on the fabrics showed no significant effects on the aPDI results, and furthermore, appeared to provide the photosensitizer with some measure of protection from photobleaching, thus improving the photostability of the dual-dyed fabrics. Taken together, these results suggest the feasibility of low cost, scalable and color variable thionine-conjugated TC blended fabrics as potent self-disinfecting textiles.

Nanomaterial-enhanced 3D-printed sensor platform for simultaneous detection of atrazine and acetochlor

Biosens Bioelectron 2021 Jul 15;184:113238.PMID:33878594DOI:10.1016/j.bios.2021.113238.

The widespread use of herbicides in agriculture and gardening causes environmental and safety issues such as water pollution. Thus, efficient and convenient analysis of the levels of herbicide residues is of significant importance. Here, we employed 3D-printing to design a multiplex immunosensor for simultaneous detection of two widely used herbicides, atrazine and acetochlor. Multiplexing was achieved through customization of a lateral flow immunoassay, and then integrated with an electrochemical analyzer for ultrasensitive detection. Quantification of herbicide residues was realized through the detection of a novel nanomaterial label, the mesoporous core-shell palladium@platium nanoparticle (Pd@Pt NP), for its outstanding peroxidase-like property. During the electrochemical analysis, the catalytic activity of Pd@Pt NPs on the redox reaction between Thionin acetate and hydrogen peroxide provided an electrochemically driven signal that accurately indicated the level of herbicide residues. Using this Nanomaterial-enhanced multiplex electrochemical immunosensing (NEMEIS) system, simultaneous detection of atrazine and acetochlor was realized with a limit of detection of 0.24 ppb and 3.2 ppb, respectively. To further evaluate the feasibility, the optimized NEMEIS was employed for detection in atrazine and acetochlor residue-containing spiked samples, and an overall recovery with 90.8% - 117% range was obtained. The NEMEIS constructed with the aid of 3D-printing provides a rapid, precise, economical, and portable detection device for herbicides, and its success suggests potential broad applications in chemical analysis, biosensors and point-of-care monitoring.

Photocatalytic dye-degradation activity of nano-crystalline Ti1- x M x O2- δ (M =Ag, Pd, Fe, Ni and x = 0, 0.01) for water pollution abatement

RSC Adv 2022 Jun 28;12(29):18794-18805.PMID:35873333DOI:10.1039/d2ra02847f.

Nanocrystalline metal-ion (M = Fe, Ni, Ag, and Pd) doped and undoped anatase-TiO2 powders were prepared using a solution combustion method. The photocatalytic degradation of different dyes such as methylene blue (MB), rhodamine B (RB), rhodamine B base (RBB), and Thionine acetate (TA) was investigated under UV exposure. The degradation rate of the dyes were found to be better in the case of Ag+ and Pd2+ doped TiO2, whereas Fe3+ and Ni2+ doped TiO2 showed lower photocatalytic activity compared to undoped TiO2 nanoparticles. Combustion synthesized catalysts exhibited much better activity compared to the commercial Degussa P25 (75% anatase + 25% rutile) TiO2 photocatalyst. The intermediate states created in the band gap of the TiO2 photocatalyst due to doping of first row transition metal ions (such as Fe3+ and Ni2+) into the TiO2 lattice act as recombination centres and the electrons present in the d-orbital quench the photogenerated holes by indirect recombination, hence increasing e--h+ recombination rates. As a result, a decrease in the photocatalytic activity of TiO2 doped with first row transition metal ions is observed. However, in the case of noble metal ions (such as Ag+ and Pd2+) in TiO2, photoreduction of Ag+ and Pd2+ ions occurs upon UV irradiation, hence the noble metal-ions act as electron scavengers. Consequently, the lifetime of the holes (h+) increases and hence higher photocatalytic oxidation activity of the dyes is observed. A novel strategy of electron scavenging is envisaged here to develop Ag+ and Pd2+ doped TiO2 to increase the photocatalytic oxidation of organic dyes for the development of better water pollution abatement catalysts. Redox-pair stabilization in the TiO2 lattice similar to photo-chromic glasses play a defining role in enhancing the photocatalytic activity of the catalyst and is a key finding for the development of superior photocatalysts. With the help of UV-vis and fluorescence spectroscopy, the mechanisms of the superior oxidation activity of Pd2+ and Ag+ doped TiO2 nanoparticles are explained.