Tetracycline Hydrochloride
(Synonyms: 盐酸四环素) 目录号 : GC15020
A selective antibiotic used in cell culture systems
Cas No.:64-75-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Tetracycline (hydrochloride) is a broad-spectrum antibiotic, exhibiting activity against a wide range of gram-positive and gram-negative bacteria.
Tetracyclines are broad-spectrum agents, exhibiting activity against a wide range of gram-positive and gram-negative bacteria, atypical organisms such as chlamydiae, mycoplasmas, and rickettsiae, and protozoan parasites. Tetracyclines inhibit bacterial protein synthesis by preventing the association of aminoacyl-tRNA with the bacterial ribosome. Tetracyclines traverse the outer membrane of gram-negative enteric bacteria through the OmpF and OmpC porin channels, as positively charged cation (probably magnesium)-tetracycline coordination complexes [1].
The tetracyclines have applications for the treatment of infections in poultry, cattle, sheep, and swine. In some cases, e.g., for therapeutic treatment of large numbers of poultry reared on commercial farms, the antibiotics are added directly to feed or water or can be administered in aerosols. Tetracyclines could be used as growth promotion or growth enhancement. Tetracyclines are used in aquaculture to control infections in salmon, catfish, and lobsters[2].
References:
[1]. Chopra I, et al.Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology ofbacterial resistance.Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60
| Cas No. | 64-75-5 | SDF | |
| 别名 | 盐酸四环素 | ||
| 化学名 | (4S,4aS,5aS,6S,12aR)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide;hydrochloride | ||
| Canonical SMILES | CC1(C2CC3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.Cl | ||
| 分子式 | C22H25ClN2O8 | 分子量 | 480.90 |
| 溶解度 | ≥ 12.02 mg/mL in DMSO with gentle warming, ≥ 57.7 mg/mL in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0794 mL | 10.3972 mL | 20.7943 mL |
| 5 mM | 0.4159 mL | 2.0794 mL | 4.1589 mL |
| 10 mM | 0.2079 mL | 1.0397 mL | 2.0794 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Controlled release of Tetracycline Hydrochloride from poly(蠅-pentadecalactone-co-蔚-caprolactone)/gelatin nanofibers
Eur J Pharm Biopharm 2021 May;162:59-69.33727142 10.1016/j.ejpb.2021.02.009
Development of drug delivery systems is an extensively researched area in biomedical field. In recent years, there is an increasing interest on fabrication of biocompatible nanofibrous drug delivery systems. In the present study, poly(蠅-pentadecalactone-co-蔚-caprolactone)/gelatin nanofibrous membranes were fabricated for the controlled delivery and release of Tetracycline Hydrochloride (TCH) antibiotic. Poly(蠅-pentadecalactone-co-蔚-caprolactone) content provides an originality to the membrane, since it has been synthesized enzymatically previously. Varied amounts of Tetracycline Hydrochloride including poly(蠅-pentadecalactone-co-蔚-caprolactone)/gelatin (1:1, v:v) binary polymer blend was electrospun and characterizations (morphological and molecular structure, wettability characteristics, and thermal behavior) were applied to investigate the incorporation of drug molecule. Afterwards, in vitro drug release studies were carried out and mathematical modelling was applied to drug release data in order to clarify the transport mechanism of drug. TCH release profile comprised of an initial burst release in first hour and followed by a sustained release through 14 days which allowed sufficient antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. The presented drug delivery system may be applied as an antibacterial wound dressing device for skin infections.
Tetracycline hydrochloride-stressed succession in microbial communities during aerobic composting: Insights into bacterial and fungal structures
Chemosphere 2022 Feb;289:133159.34871611 10.1016/j.chemosphere.2021.133159
Available information that whether antibiotics affect the succession in microbial communities during aerobic composting remains limited. Thus, this work investigated the dynamic changes in bacterial and fungal structures during aerobic composting amended with Tetracycline Hydrochloride (TCH: 0, 50, 150 and 300 mg kg-1). Composting phases significantly affected bacterial and fungal communities, but only fungi strongly responded to antibiotics, while bacteria did not. Firmicutes, Proteobacteria, Bacteroidota and Actinobacteriota were primary bacterial phylum. Neocallimastigomycota was dominant fungal phylum at temperature-heating phase, then Basidiomycota and Ascomycota became main fungal phylum at thermophilic and temperature-colling phases. Low TCH concentration promoted Chytridiomycota growth, while high TCH concentration inhibited mostly fungal activity in TCH-amended composting. Nitrogen species were critical factors controlling the succession in bacterial and fungal communities during composting process. These results cast a new light on understanding about microbial function during aerobic composting.
Efficient Adsorption-Photocatalytic Removal of Tetracycline Hydrochloride over Octahedral MnS
Int J Mol Sci 2022 Aug 19;23(16):9343.36012607 PMC9408993
To disclose the effect of crystal plane on the adsorption-photocatalytic activity of MnS, octahedral MnS was prepared via the hydrothermal route to enhance the adsorption and photocatalytic efficiencies of Tetracycline Hydrochloride (TCH) in visible light region. The optimal MnS treated at 433 K for 16 h could remove 94.83% TCH solution of 260 mg L-1 within 180 min, and its adsorption-photocatalytic efficiency declined to 89.68% after five cycles. Its excellent adsorption-photocatalytic activity and durability were ascribed to the sufficient vacant sites of octahedral structure for TCH adsorption and the feasible band-gap structure for visible-light response. In addition, the band gap structure (1.37 eV) of MnS with a conduction band value of -0.58 eV and a valence band value of 0.79 eV was favorable for the generation of O2-, while unsuitable for the formation of OH. Hence, octahedral MnS was a potential material for the removal of antibiotics from wastewater.
Efficient degradation of Tetracycline Hydrochloride by photocatalytic ozonation over Bi2WO6
Chemosphere 2021 Nov;283:131256.34182642 10.1016/j.chemosphere.2021.131256
Photocatalytic ozonation technique for wastewater treatment has received much attention for their efficient capability in the mineralization of persistent organic pollutants. In this study, nanostructured Bi2WO6 was prepared by hydrothermal method and applied in the photocatalytic ozonation process for Tetracycline Hydrochloride (TCH) degradation under simulated solar light irradiation. Bi2WO6 triggered an effective synergy between photocatalysis and ozonation, and it showed a good activity and adaptability in the degradation of organic compounds. Besides, the influence of experimental factors on the total organic carbon removal (including catalyst dosage, ozone concentration, initial pH, reaction temperature and coexisting ions) was also investigated comprehensively. Spin-trapping electron paramagnetic resonance measurements and quenching experiments demonstrated that O2-, OH, 1O2 and h+ contributed to TCH degradation. The possible degradation pathways of TCH were proposed by identifying the intermediates with liquid chromatography-mass spectroscopy.
Characterization of Tetracycline Hydrochloride Compounded in a Miracle Mouthwash Formulation
AAPS PharmSciTech 2019 Apr 30;20(5):178.31041553 10.1208/s12249-019-1388-x
Miracle mouthwash (MMW) is a commonly prescribed oral formulation compounded with varying active ingredients, depending on purpose of treatment. Due to patient-to-patient customization, the solubility, stability, and solid-state characteristics of the active ingredients may not be known after compounding. This study found that the common antibiotic, Tetracycline Hydrochloride (HCl), compounded in MMW formulations that contained dexamethasone elixir and diphenhydramine, underwent significant physical-chemical changes. Simulated patient conditions demonstrated appreciable fluctuations from the target content of 50 mg tetracycline HCl per teaspoon over 15 days. The lowest tetracycline content sampled was 32.5 mg, while the highest content sampled was 53.0 mg. Although tetracycline HCl went into solution after compounding, tetracycline did not remain in solution. In fact, the amount of tetracycline in solution declined exponentially, with over two-thirds of tetracycline precipitating out within the first day of compounding and 14% remaining in solution after 15 days. Crystals that formed within the MMW formulation were analyzed using differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and powder X-ray diffraction (PXRD), which confirmed a solvent-mediated phase transformation of tetracycline HCl to tetracycline hexahydrate. For tetracycline in solution, pH had a significant effect on chemical degradation. Therefore, tetracycline HCl compounded in MMW formulations can have significant physical-chemical stability changes, possibly impacting patient dosing.