| S Tag Peptide 目录号 GP10155 |
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
S Tag Peptide Dilution Calculator
S Tag Peptide Molarity Calculator
| Cas No. | N/A | SDF | |
| 别名 | H-Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser-OH | ||
| 化学名 | S Tag | ||
| Canonical SMILES | CC(C(C(=O)NC(C)C(=O)NC(C)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CC1=CC=CC=C1)C(=O)NC(CCC(=O)O)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCC(=O)N)C(=O)NC(CC2=CN=CN2)C(=O)NC(CCSC)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(CCCCN)N)O | ||
| 分子式 | C73H117N23O25S | 分子量 | 1748.91 |
| 溶解度 | ≥174.9mg/mL in DMSO, <2.5 mg/mL in EtOH, ≥50 mg/mL in H2O | 储存条件 | Desiccate at -20°C |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。溶液形式可以在-20℃下储存几个月。 | ||
| 运输条件 | 所有可选规格：常温运输或根据产品储存温度要求用蓝冰运输。 | ||
S Tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, it is called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.
It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies. [1]
References:
1. R.T. Raines et al., The S-Tag Fusion System for Protein Purification. Methods Enzymol. 326, 362-367 (2000)