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S-(5'-Adenosyl)-L-methionine chloride (hydrochloride)

(Synonyms: 5'-[[(3S)-3-氨基-3-羧基丙基]甲基锍]-5'-脱氧腺苷氯化物二盐酸盐) 目录号 : GC44859

Ubiquitous methyl donor

S-(5'-Adenosyl)-L-methionine chloride (hydrochloride) Chemical Structure

Cas No.:86867-01-8

规格 价格 库存
1mg
¥565.00
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2.5mg
¥1,250.00
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5mg
¥2,228.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

S-(5'-Adenosyl)-L-methionine chloride (SAM) is a ubiquitous methyl donor involved in a wide variety of biological reactions, including those mediated by DNA and protein methyltransferases. The transfer of a methyl group from SAM to an acceptor produces S-adenosyl-L-homocysteine . SAM is also metabolized by SAM decarboxlase to give decarboxylated SAM, which is involved in the polyamine pathway that generates spermine. Chemical's SAM has been purified to remove all S-adenosylhomocysteine, a known inhibitor of most SAM-dependent methyltransferases which is commonly found in other commercially available SAM. In addition, 's SAM contains no S-methylthioadenosine, a common impurity which may act as a substrate in some assay formats, resulting in high background.

Chemical Properties

Cas No. 86867-01-8 SDF
别名 5'-[[(3S)-3-氨基-3-羧基丙基]甲基锍]-5'-脱氧腺苷氯化物二盐酸盐
Canonical SMILES NC1=C2C(N([C@@H]3O[C@H](C[S+](CC[C@H](N)C(O)=O)C)[C@@H](O)[C@H]3O)C=N2)=NC=N1.[Cl-].Cl.Cl
分子式 C15H23ClN6O5S•2HCl 分子量 507.8
溶解度 Methanol: 1 mg/ml,Water: 5 mg/ml 储存条件 Store at -80°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.9693 mL 9.8464 mL 19.6928 mL
5 mM 0.3939 mL 1.9693 mL 3.9386 mL
10 mM 0.1969 mL 0.9846 mL 1.9693 mL
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Research Update

A sensitive UPLC-MS/MS method for simultaneous quantification of one-carbon metabolites & co-factors in human plasma

J Pharm Biomed Anal 2022 Sep 20;219:114944.PMID:35863169DOI:10.1016/j.jpba.2022.114944

One-carbon metabolism is an important metabolic pathway involved in many diseases, such as congenital malformations, tumours, cardiovascular diseases, anaemia, depression, cognitive diseases and liver disease. However, the current methods have specific defects in detecting and qualifying the related compounds of one-carbon metabolism. In this study, a validated method was established to simultaneously quantify 22 one-carbon metabolites & co-factors in human plasma and applied to the study of correlation between one-carbon metabolism and colorectal cancer in human plasma samples, which were from 44 healthy subjects and 55 colorectal cancer patients. The method used ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS), and the analytes included betaine, L-carnitine, L-cystathionine, L-cysteine, dimethylglycine, DL-homocysteic acid, homocysteine, methionine, pyridoxal hydrochloride, pyridoxamine dihydrochloride, pyridoxine dihydrochloride, S-(5'-Adenosyl)-L-homocysteine, serine, choline chloride, folic acid, glycine, pyridoxal phosphate monohydrate, riboflavin, taurine, 5-methyltetrahydrofolate, S-(5'-adenosyl)-L-methionine disulfate salt, trimethylamine oxide. The developed method was successfully applied to the quantification of 22 one-carbon metabolites & co-factors in human plasma from colorectal cancer patients and healthy individuals. The plasma concentrations of dimethylglycine was significantly decreased in the patients compared with the healthy individuals, while L-cystathionine was increased.